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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3933-50 50 µg (0.5 mg/ml) $425 
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody (0.01µg/ml) staining of Human (A) and (0.3ug/ml) Rat (B) Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ACTA2 / Smooth Muscle Actin Antibody - Antibody staining (0.1 ug/ml) of Human Duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ACTA2 / Smooth Muscle Actin Antibody - ACTA2 / Smooth Muscle Actin antibody (0.01µg/ml) staining of Human (A) and (0.3ug/ml) Rat (B) Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ACTA2 / Smooth Muscle Actin Antibody - Antibody staining (0.1 ug/ml) of Human Duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
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PathPlus™ ACTA2 / Smooth Muscle Actin Antibody aa1‑50 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3933

PathPlus™ ACTA2 / Smooth Muscle Actin Antibody aa1‑50 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3933

Note: This antibody replaces LS-C54442
Antibody:
Goat Polyclonal to Human ACTA2 / Smooth Muscle Actin
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3933-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ACTA2 / Smooth Muscle Actin
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
ACTA2 / Smooth Muscle Actin (SMA) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. In immunohistochemistry, ACTA2 is to define smooth muscle cells and myofibroblasts in normal and malignant tissues. It is a common myoepithelial marker for normal and cancerous breast, salivary and sweat gland tissue. High levels of ACTA2 expression are correlated with aggressiveness, invasion and poorer prognosis in breast cancers positive for EGFR and ERBB2, basal cell carcinoma, and pancreatic ductal adenocarcinoma. ACTA2 can also be used to differentiate pleuropulmonary desmoid tumors (positive) from solitary fibrous tumors (negative). ACTA2 has membranous and cytoplasmic staining, and in normal tissue it is positive in smooth muscle cells, breast myoepithelial cells, chrondrocytes, decidual stromal cells, myofibroblasts, osteoblasts, pericytes, and salivary and sweat gland cells. Pancreas. 2010 Nov;39(8):1254-1262, PMID: 20467342; Oncotarget 2016 Jul; 8.31; 50570-50581, PMID: 28881584; Hum Pathol. 2010 Aug;41(8):1128-37, PMID: 20381122; Arch Pathol Lab Med 2006;130:1503, PMID: 17090192
Target
Human ACTA2 / Smooth Muscle Actin
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to the N-terminus of the human Alpha-Smooth Muscle Actin (ACTA2).
Blocking Peptide
LS-E25755 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3933. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-50
Specificity
Human ACTA2. Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA2 / Smooth Muscle Actin
P62736 NM_001613 NP_001604.1

Validation

ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Publications (2)

Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response. Westendorp BF, Büller NVJA, Karpus ON, van Dop WA, Koster J, Versteeg R, Koelink PJ, Snel CY, Meisner S, Roelofs JJTH, Uhmann A, Ver Loren van Themaat E, Heijmans J, Hahn H, Muncan V, Wildenberg ME, van den Brink GR,. Cellular and molecular gastroenterology and hepatology. 2017 5:67-82. (WB; Human)

Towards an in vitro fibrogenesis model of human vocal fold scarring. Graupp M, Rinner B, Frisch MT, Weiss G, Fuchs J, Sundl M, El-Heliebi A, Moser G, Kamolz LP, Karbiener M, Gugatschka M. European Archives of Oto-Rhino-Laryngology and Head & Neck. 2018 May;275:1211-1218. (Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/14/2019