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Catalog Number Size Price
LS-B16606-100 100 µl (1 mg/ml) $460 
ACTA2 / Smooth Muscle Actin Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - ICC/IF analysis of ACTA2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ACTA2 / Smooth Muscle Actin Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: SW480 cell lysate Lane 3.: HeLa cell lysate
ACTA2 / Smooth Muscle Actin Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
ACTA2 / Smooth Muscle Actin Antibody - ICC/IF analysis of ACTA2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ACTA2 / Smooth Muscle Actin Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: SW480 cell lysate Lane 3.: HeLa cell lysate
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PathPlus™ Monoclonal Mouse anti‑Human ACTA2 / Smooth Muscle Actin Antibody (clone AT132D3, IHC, IF, WB) LS‑B16606

PathPlus™ Monoclonal Mouse anti‑Human ACTA2 / Smooth Muscle Actin Antibody (clone AT132D3, IHC, IF, WB) LS‑B16606

Note: This antibody replaces LS-C755379
Antibody:
ACTA2 / Smooth Muscle Actin Mouse anti-Human Monoclonal (AT132D3) Antibody
Application:
IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16606-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ACTA2 / Smooth Muscle Actin Mouse anti-Human Monoclonal (AT132D3) Antibody
Application:
IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ACTA2 / Smooth Muscle Actin (SMA) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. In immunohistochemistry, ACTA2 is to define smooth muscle cells and myofibroblasts in normal and malignant tissues. It is a common myoepithelial marker for normal and cancerous breast, salivary and sweat gland tissue. High levels of ACTA2 expression are correlated with aggressiveness, invasion and poorer prognosis in breast cancers positive for EGFR and ERBB2, basal cell carcinoma, and pancreatic ductal adenocarcinoma. ACTA2 can also be used to differentiate pleuropulmonary desmoid tumors (positive) from solitary fibrous tumors (negative). ACTA2 has membranous and cytoplasmic staining, and in normal tissue it is positive in smooth muscle cells, breast myoepithelial cells, chrondrocytes, decidual stromal cells, myofibroblasts, osteoblasts, pericytes, and salivary and sweat gland cells. References: Pancreas. 2010 Nov;39(8):1254-1262, PMID: 20467342; Oncotarget 2016 Jul; 8.31; 50570-50581, PMID: 28881584; Hum Pathol. 2010 Aug;41(8):1128-37, PMID: 20381122; Arch Pathol Lab Med 2006;130:1503, PMID: 17090192
Target
Human ACTA2 / Smooth Muscle Actin
Synonyms
ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin, aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
AT132D3
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Recombinant human ACTA2 (3-377aa) purified from E. coli
Applications
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA2 / Smooth Muscle Actin
P62736 NM_001613 NP_001604.1

Validation

ACTA2 / Smooth Muscle Actin Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

ACTA2 / Smooth Muscle Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

ACTA2 / Smooth Muscle Actin Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ ACTA2 / Smooth Muscle Actin Antibody (clone AT132D3) for IHC, ICC, IF/Immunofluorescence, WB/Western, ELISA LS-B16606 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/18/2024