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order histories, retained contact details for faster checkout, review submissions, and special promotions.
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Immunohistochemistry Services
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.
TCR Screening ServicesTest your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy
- Details about how to buy our products.
Orders@LSBio.com
- To submit a new order.
Customer.Support@LSBio.com
- To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com
- To request technical information about an LSBio product or its applications
Sales@LSBio.com
- To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
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- To find your local distributor if you're not within the United States.
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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
LSBio DNA-Binding ELISA Kits
DNA-Binding ELISA Kits enable researchers to quickly and easily detect active transcription factors in eukaryotic nuclear extracts or cell lysates without the use of harmful radioactive reagents.
Additionally our Phospho-DNA Binding kits can be used to study the effects of phosphorylation on transcription factor activation.
Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample extraction to analysis of the results.
DNA-Binding kits are available for both the detection of both phosphorylated and non-phosphorylated targets.
Features:
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Kit Components:
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Features:
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Kit Components:
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DNA-Binding ELISA Protocol
| 1) The 96-well microtiter plate comes pre-bound with specific double-stranded (dsDNA) oligonucleotides. |
| 2) Following a blocking step samples are added to each well and transcription factor binds to the oligonucleotides. |
| 3) The wells are washed and primary antibody is added which binds activated transcription factor. |
| 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
| 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
Phospho-Specific DNA-Binding ELISA Kit Protocol
| 1) The 96-well microtiter plate comes pre-bound with specific double-stranded (dsDNA) oligonucleotides. |
| 2) Following a blocking step samples are added to each well and phosphorylated transcription factor binds to the oligonucleotides. |
| 3) The wells are washed and a phospho-specific primary antibody is added which binds activated transcription factor. |
| 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
| 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
The plate can then be read in a spectrophotometer at a wavelength of 450 nm.
