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Catalog Number Size Price
LS-B2506-50 50 µl (0.2 mg/ml) $515 
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC‑plus™ Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (N‑Terminus, IHC) LS‑B2506

IHC‑plus™ Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (N‑Terminus, IHC) LS‑B2506

Note: This antibody replaces LS-C34848
Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B2506-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Smooth Muscle Actin antibody LS-B2506 is an unconjugated rabbit polyclonal antibody to Smooth Muscle Actin (ACTA2) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human ACTA2 / Smooth Muscle Actin
Synonyms
ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin, aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. Cellular Localization: Cytoplasmic
Epitope
N-Terminus
Specificity
Recognizes smooth muscle Actin. Reacts with tumors arising from smooth muscles and myoepithelial cells. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Species cross-reactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2506 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2506 was determined to be 5 ug/ml. Positive control: Smooth muscle of arteries, gut wall, uterus and leiomyosarcoma.
Presentation
10 mM PBS, pH 7.6, 15 mM Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA2 / Smooth Muscle Actin
P62736 NM_001613 NP_001604.1

Validation

ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ACTA2 antibody IHC of human colon, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ACTA2 antibody IHC of human colon, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ACTA2 / Smooth Muscle Actin Antibody (N-Terminus) for IHC LS-B2506 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/28/2024