LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

LSBio Cell-Based ELISA Kits

ELISA Kit Image
Cell-Based ELISA kits are a fast and cost-effective way to quantify specific target antigens in vitro. Additionally, phospho-specific cell-based ELISA kits enable researchers to quickly evaluate changes in the phosphorylation state of specific proteins in whole cells under various treatment conditions. In a single experiment the activation state of 96 treated and untreated cells can be evaluated simultaneously. Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results. Cell-Based ELISA kits are available for both the detection of both phosphorylated and non-phosphorylated targets.




Features:

  • Ready-to-use kit includes all necessary reagents
  • Direct in vitro detection on un-lysed cultured cells
  • Phospho- and non-Phospho specific kits available
  • Excellent sensitivity, specificity, and reproducibility
  • Built on standard 96-well microtiter format
  • 450 nm colorimetric detection

Kit Components:

  • 96-well microtiter plate
  • Fixing Solution
  • Quenching buffer
  • Blocking Buffer
  • Wash Buffers
  • Primary Detection Antibody
  • HRP-Conjugated Secondary Antibody
  • TMB Substrate Solution
  • Stop Buffer
  • Plate Sealer Sheets

Cell-Based ELISA Kit Protocol

Cell-Based Overview
1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. The cells are then fixed and blocked. 2) Antigen specific primary antibody is added and binds to the target antigen on the cells surface. 3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. 4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow.


Phospho-Specific Cell-Based ELISA Kit Protocol

Cell-Based Phosphorylation Overview
1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. to induce expression of the phosphorylated target. The cells are then fixed and blocked. 2) Phospho-specific or pan-specific primary antibody is added and binds to the target antigen on the cells surface. 3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. 4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow.

The plate can then be read in a spectrophotometer at a wavelength of 450 nm.

Phosphorylation Standard Curve ELISA Standard Curve ELISA Plate With TMB Substrate

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number