LSBio Cell-Based ELISA Kits
Cell-Based ELISA kits are a fast and cost-effective way to quantify specific target antigens in vitro.
Additionally, phospho-specific cell-based ELISA kits enable researchers to quickly evaluate changes in the phosphorylation state of specific proteins in whole cells under various treatment conditions.
In a single experiment the activation state of 96 treated and untreated cells can be evaluated simultaneously.
Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.
Cell-Based ELISA kits are available for both the detection of both phosphorylated and non-phosphorylated targets.
Features:
- Ready-to-use kit includes all necessary reagents
- Direct in vitro detection on un-lysed cultured cells
- Phospho- and non-Phospho specific kits available
- Excellent sensitivity, specificity, and reproducibility
- Built on standard 96-well microtiter format
- 450 nm colorimetric detection
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Kit Components:
- 96-well microtiter plate
- Fixing Solution
- Quenching buffer
- Blocking Buffer
- Wash Buffers
- Primary Detection Antibody
- HRP-Conjugated Secondary Antibody
- TMB Substrate Solution
- Stop Buffer
- Plate Sealer Sheets
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Cell-Based ELISA Kit Protocol
| 1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. The cells are then fixed and blocked. |
2) Antigen specific primary antibody is added and binds to the target antigen on the cells surface. |
3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
Phospho-Specific Cell-Based ELISA Kit Protocol
| 1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. to induce expression of the phosphorylated target. The cells are then fixed and blocked. |
2) Phospho-specific or pan-specific primary antibody is added and binds to the target antigen on the cells surface. |
3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
The plate can then be read in a spectrophotometer at a wavelength of 450 nm.
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