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order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
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Immunohistochemistry Services
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.
TCR Screening ServicesTest your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy
- Details about how to buy our products.
Orders@LSBio.com
- To submit a new order.
Customer.Support@LSBio.com
- To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com
- To request technical information about an LSBio product or its applications
Sales@LSBio.com
- To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List
- To find your local distributor if you're not within the United States.
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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
LSBio Cell-Based ELISA Kits
Cell-Based ELISA kits are a fast and cost-effective way to quantify specific target antigens in vitro.
Additionally, phospho-specific cell-based ELISA kits enable researchers to quickly evaluate changes in the phosphorylation state of specific proteins in whole cells under various treatment conditions.
In a single experiment the activation state of 96 treated and untreated cells can be evaluated simultaneously.
Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.
Cell-Based ELISA kits are available for both the detection of both phosphorylated and non-phosphorylated targets.
Features:
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Kit Components:
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Features:
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Kit Components:
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Cell-Based ELISA Kit Protocol
| 1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. The cells are then fixed and blocked. |
| 2) Antigen specific primary antibody is added and binds to the target antigen on the cells surface. |
| 3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
| 4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
Phospho-Specific Cell-Based ELISA Kit Protocol
| 1) Cell monolayers are cultured in the 96-well microtiter plate. Each well can then be treated with stimulants, inhibitors, etc. to induce expression of the phosphorylated target. The cells are then fixed and blocked. |
| 2) Phospho-specific or pan-specific primary antibody is added and binds to the target antigen on the cells surface. |
| 3) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
| 4) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
The plate can then be read in a spectrophotometer at a wavelength of 450 nm.
