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Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
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LSBio Assay Kits

Assay Kit Image
LSBio’s growing collection of Assay kits provide researchers with the tools necessary to monitor various biological processes in the course of their life science research, drug discovery & development, or environmental monitoring studies. Assay kits are available for monitoring cell proliferation, damage, and oxidative stress, cytotoxicity, apoptosis, metabolism, signaling pathways and signal transduction. Each kit includes all of the necessary reagents to conduct the assay, and a clear, detailed protocol. LSBio’s experienced technical staff is also available to address any questions researchers may have about each kit and how they may be used to generate optimal results.



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Glucose Assay Kit - Measurement of Glucose levels in human serum (1 µl of 1:10 diluted) & rat tissue lysates from liver, kidney & muscle (0.14 µg, 0.19 µg & 0.93 µg respectively).
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric)
100asy   $580.00
Glucose Assay Kit
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Detection/Quantition
Glucose
Spectrophotometry (visible)
100tst   $285.00
Glucose Assay Kit
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric), Spectrophotometry (visible)
100tst   $320.00
Glucose Assay Kit - A) Glucose Uptake time course, Jurkat Cells: Cells were starved (Glucose-free, FBS-free media), inhibitor incubation time: 2 hours. B) 3T3-L1 cells were Glucose and FBS Deprived for 24 hours, switched to media with Glucose, stimulated without or with Insulin (10 ng/ml) for 15 min and with or without 1X Inhibitor. C) HeLa cells were Glucose and FBS deprived for 2 hours, then switched to Glucose and FBS-free media (Control), or complete media (10% FBS) with or without 1X Inhibitor for 30 min.
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric)
50asy   $800.00
Glucose Assay Kit - (a), (b) and (c) 2-DG uptake in human adipocytes, Hela Cells and 3T3-L1 cells respectively. Assays were performed following kit protocol. I = Insulin; P = Phloretin.
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric)
100asy   $1,160.00
Glucose Assay Kit - 2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.
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Detection/Quantition
Glucose
Spectrophotometry (visible)
100asy   $1,210.00
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were pretreated with or without 4 µl phloretin (1X concentration) for 45 min. After pretreatment, cells were washed and incubated with 2-NBDG Reagent, Glucose Uptake Enhancer, and the same concentration of phloretin for another 30 min. according to the kit’s protocol. Comparison of histograms from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
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Detection/Quantition
Glucose
Flo, Microscopy (fluorescent)
50asy   $555.00
Glucose Assay Kit - Standard Curve for Glucose run using the kit protocol.
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Detection/Quantition
Glucose
Spectrophotometry (visible)
100asy   $540.00
Glucose Assay Kit - Glucose uptake in Jurkat and HeLa cells. 2.5E5 Jurkat cells were treated with or without 4 µl Phloretin (1X concentration) for 45 min. After treatment, cells were washed and incubated with Glucose Tracker Reagent, Glucose Tracker Enhancer, and the same concentration of Phloretin for another 30 min. according to the kit protocol. Comparison of histogram from flow analysis showing the inhibition of glucose uptake by Phloretin in Jurkat cells (Black: negative control cells; Orange: in the presence of Phloretin; Blue: without Phloretin).
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Detection/Quantition
Glucose
Flo, Microscopy (fluorescent)
50asy   $1,160.00
Glucose Assay Kit - Quantitation of Glucose in human serum. Serum samples were deproteinized using a 10kDa Spin Column (10,000 x g, 10 min, 4°C). Undiluted serum filtrate (1 µl) samples were added to the wells directly. Calculated concentration: 86.78 ± 0.1 mg/dl.
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384 Well HTS Screen
Glucose
Spectrophotometry (visible)
384asy   $600.00
Glucose Assay Kit - Quantitation of Glucose in human urine & serum. Urine & serum samples were deproteinized using a 10 kDa Spin Column (10000xg, 10 min, 4°C). Urine filtrate (20 µl) & serum filtrate (1 µl) were spiked with a known amount of glucose as internal standard (4 nmol). Urine: 3.00 ± 0.4 mg/dl; Serum: 44.2 ± 6.7 mg/dl.
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric), Spectrophotometry (visible)
100asy   $540.00
Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
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Detection/Quantition
Glucose
Spectrophotometry (fluorometric)
100tst   $635.00
Glucose Assay Kit
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Detection/Quantition
Glucose
Spectrophotometry (visible)
100tst   $320.00
Glucose Assay Kit
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Detection/Quantition
Glucose
Spectrophotometry (visible)
100tst   $320.00
ADA / Adenosine Deaminase Assay Kit - ADA specific activity in rat brain lysate and Jurkat cell lysate.
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Enzymatic Activity Assay
ADA / Adenosine Deaminase
Spectrophotometry (UV)
100asy   $445.00
ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
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Enzymatic Activity Assay
ADA / Adenosine Deaminase
Spectrophotometry (fluorometric)
100asy   $480.00
ACHE / Acetylcholinesterase Assay Kit - AChE activity in serum (1 µl) & Positive Control (1 µl).
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Enzymatic Activity Assay
ACHE / Acetylcholinesterase
Spectrophotometry (visible)
100asy   $580.00
ACHE / Acetylcholinesterase Assay Kit
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Detection/Quantition
ACHE / Acetylcholinesterase
Spectrophotometry (visible)
100tst   $395.00
Fructose Assay Kit
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Detection/Quantition
Fructose
Spectrophotometry (visible)
100tst   $370.00
Fructose Assay Kit - Fructose in beverages: Apple Cider or Cola from a soda fountain was diluted 1:200 with dH2O, and 2-10 µl of the dilutions were assayed using the kit protocol. Values were averaged to give presented data.
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Detection/Quantition
Fructose
Spectrophotometry (visible)
100asy   $525.00
Fructose Assay Kit - Fructose Assay Kit standard curve.
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Detection/Quantition
Fructose
Spectrophotometry (fluorometric)
100asy   $565.00
Fructose Assay Kit - Fructose Assay Kit standard curve.
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Detection/Quantition
Fructose
Spectrophotometry (fluorometric), Spectrophotometry (visible)
100asy   $525.00
Superoxide Dismutase Assay Kit
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Detection/Quantition
Superoxide Dismutase
Spectrophotometry (visible)
100tst   $295.00
Superoxide Dismutase Assay Kit - SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
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Enzymatic Activity Assay
Superoxide Dismutase
Spectrophotometry (visible)
100asy   $465.00
Lactate Assay Kit
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Detection/Quantition
Lactate
Spectrophotometry (visible)
100tst   $385.00
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