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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LSBio Assay Kits

Assay Kit Image
LSBio’s growing collection of Assay kits provide researchers with the tools necessary to monitor various biological processes in the course of their life science research, drug discovery & development, or environmental monitoring studies. Assay kits are available for monitoring cell proliferation, damage, and oxidative stress, cytotoxicity, apoptosis, metabolism, signaling pathways and signal transduction. Each kit includes all of the necessary reagents to conduct the assay, and a clear, detailed protocol. LSBio’s experienced technical staff is also available to address any questions researchers may have about each kit and how they may be used to generate optimal results.


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Colorimetric (240 nm Absorbance) (1)
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Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/587 nm) (28)
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FACS (488 nm excitation laser), Fluorescent (excitation range 420-495 nm) (2)
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Fluorometric (Ex/Em 535/587 nm), Colorimetric (570 nm Absorbance) (23)
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Western blot (135)
Target
Detection Type
Showing 1-25 of 887 results

HAT Activity Assay Kit (Colorimetric) - LS-K20
Quantitative Assay Kit
Histone Acetyltransferase
Colorimetric (440 nm Absorbance)
100assy  $525.00
Analyses of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate.
Analyses of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate.

HAT (H4) Activity Assay Kit (Fluorometric) - LS-K424
Quantitative Assay Kit
Histone Acetyltransferase
Fluorometric (Ex/Em 535/587 nm)
100assy  $465.00

Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric) - LS-K93
Quantitative Assay Kit
Histone Acetyltransferase
Fluorometric (Ex/Em 535/587 nm)
100assy  $525.00
(a) HAT Activity in HeLa Nuclear Extract.  (b) Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF.
(a) HAT Activity in HeLa Nuclear Extract.  (b) Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF.

Dihydrofolate Reductase Activity Kit (Colorimetric) - LS-K3
Quantitative Assay Kit
DHFR
Colorimetric (340 nm Absorbance)
100assy  $500.00
Dihydrofolate Reductase activity in rat liver (100 µg).
Dihydrofolate Reductase activity in rat liver (100 µg).

Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric) - LS-K392
Quantitative Assay Kit
DHFR
Colorimetric (340 nm Absorbance)
100asy  $590.00

Superoxide Dismutase (SOD) Activity Assay Kit (Colorimetric) - LS-K25
Quantitative Assay Kit
Superoxide Dismutase
Colorimetric (450 nm Absorbance)
100assy  $440.00
SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.
SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

Superoxide Dismutase (SOD) Assay Kit (Colorimetric) - LS-K224
Quantitative Assay Kit
Superoxide Dismutase
Colorimetric (440 nm Absorbance)
100tst  $270.00
Colorimetry Image
Colorimetry Image

Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric) - LS-K31
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Colorimetric (540 nm Absorbance)
100assy  $500.00
(a) Measurement of NOS Positive Control activity (10 µl), and (b) Detection of endogenous NOSactivity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
(a) Measurement of NOS Positive Control activity (10 µl), and (b) Detection of endogenous NOSactivity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric) - LS-K53
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Fluorometric (Ex/Em 360/450 nm)
100assy  $500.00
(a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.
(a) Detection of endogenous NOS activity in135 µg of J774 and 157 µg of HeLa cells and (b) detection of endogenous NOS activity in J774.1A cell lysate (225 µg) stimulated with or without 200 ng/ml LPS and 100 ng/ml murine IFN-gamma.

Nitric Oxide Synthase (NOS) Assay Kit (Colorimetric) - LS-K196
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Colorimetric (540 nm Absorbance)
100tst  $385.00

Nitric Oxide (NO) Synthase Inhibitor Screening Kit (Colorimetric) - LS-K327
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Colorimetric (540 nm Absorbance)
100tst  $385.00
Colorimetry Image
Colorimetry Image

Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric) - LS-K102
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Fluorometric (Ex/Em 360/450 nm)
100assy  $500.00
Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.
Inhibition of NOS Enzyme activity by Diphenyleneiodonium chloride. IC50 of Diphenyleneiodonium chloride was determined to be 20 nM.

Intracellular Nitric Oxide Synthase (NOS) Detection Kit (Fluorometric) - LS-K101
Quantitative Assay Kit
NOS / Nitric Oxide Synthase
Fluorometric (Ex/Em 485/530 nm)
100asy  $400.00
NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C.  A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.  B) Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (b) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.
NOS detection in Macrophages (J774A.1): Macrophages were cultured overnight and treated the next day with either vehicle control (No treatment) or LPS (200 ng/ml) and ?-INF (100 ng/ml) for 24 hrs. After washing with Assay Buffer, the cells were stained with the Staining Dye for 1h at 37°C.  A) Detection with a plate reader: The fluorescence signal was measured at Ex/Em = 485/530 nm. Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.  B) Detection with a fluorescent microscope: The cells were washed with Assay buffer and imaged using Nikon TiE microscope. (a) and (b) control cells (vehicle treated) (c) and (d) cells treated with LPS (200 ng/ml) and ?-INF (100 ng/ml). Treatment with LPS and ?-INF induced intracellular NOS in J774A.1 cells.

Ceruloplasmin Activity Assay Kit (Colorimetric) - LS-K33
Quantitative Assay Kit
CP / Ceruloplasmin
Colorimetric (560 nm Absorbance)
100assy  $540.00
Ceruloplasmin Activity Assay kinetic curves obtained from varying sample volumes of an Ammonium Sulfate precipitate of frozen pooled human serum.
Ceruloplasmin Activity Assay kinetic curves obtained from varying sample volumes of an Ammonium Sulfate precipitate of frozen pooled human serum.

Calpain Activity Assay Kit (Fluorometric) - LS-K63
Quantitative Assay Kit
Calpain
Fluorometric (Ex/Em 400/505 nm)
100assy  $590.00
Measurement of Calpain Activity  with and without Inhibitor.  Active Calpain (1 µg) was incubated at 37 °C for one hour using the Calpain Substrate (Ac-LLY-AFC) with or without 20 µM Calpain Inhibitor (Z-LLY-FMK).
Measurement of Calpain Activity  with and without Inhibitor.  Active Calpain (1 µg) was incubated at 37 °C for one hour using the Calpain Substrate (Ac-LLY-AFC) with or without 20 µM Calpain Inhibitor (Z-LLY-FMK).

Lysozyme Activity Assay Kit (Fluorometric) - LS-K52
Quantitative Assay Kit
Lysozyme
Fluorometric (Ex/Em 360/450 nm)
100assy  $480.00
Measurement of Lysozyme activity in human samples and cell cultured samples. Undiluted pooled serum (10 µl), pooled tears (15 µl), pooled saliva (30 µl) or J774 cells (15 µg), HepG2 cells (80 µg) were incubated with Lysozyme Substrate for 60 min.
Measurement of Lysozyme activity in human samples and cell cultured samples. Undiluted pooled serum (10 µl), pooled tears (15 µl), pooled saliva (30 µl) or J774 cells (15 µg), HepG2 cells (80 µg) were incubated with Lysozyme Substrate for 60 min.

CETP Activity Assay Kit II (Fluorometric) - LS-K74
Quantitative Assay Kit
CETP
Fluorometric (Ex/Em 480/511 nm)
100assy  $665.00
a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), b) Inhibition of CETP activity from rabbit serum by Torcetrapib. The assay was run for 1 hr and the IC50 was determined to be 3.56 nM
a) Measurement of CETP activity of rabbit serum (1 µl), human plasma (1 µl) or recombinant human CETP (800 ng), b) Inhibition of CETP activity from rabbit serum by Torcetrapib. The assay was run for 1 hr and the IC50 was determined to be 3.56 nM

PLTP Activity Assay Kit (Fluorometric) - LS-K108
Quantitative Assay Kit
PLTP
Fluorometric (Ex/Em 465/535 nm)
100assy  $435.00
Measurment of PLTP Activity in rabbit serum.
Measurment of PLTP Activity in rabbit serum.

PLTP Inhibitor Drug Screening Kit (Fluorometric) - LS-K109
Quantitative Assay Kit
PLTP
Fluorometric (Ex/Em 465/535 nm)
100asy  $655.00

Beta-Glucosidase Assay Kit (Colorimetric) - LS-K198
Quantitative Assay Kit
Beta-Glucosidase
Colorimetric (405 nm Absorbance)
100tst  $395.00
Colorimetry Image
Colorimetry Image

Uric Acid Assay Kit (Colorimetric) - LS-K204
Quantitative Assay Kit
Uric Acid
Colorimetric (590 nm Absorbance)
250tst  $365.00
Colorimetry Image
Colorimetry Image

Uric Acid Assay Kit (Colorimetric/Fluorometric) - LS-K112
Quantitative Assay Kit
Uric Acid
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/587 nm)
100asy  $525.00
Quantitation of Uric Acid concentration in human urine (25 µl, 50 times diluted) and serum (25 µl, undiluted).
Quantitation of Uric Acid concentration in human urine (25 µl, 50 times diluted) and serum (25 µl, undiluted).

BCG Albumin Assay Kit (Colorimetric) - LS-K205
Quantitative Assay Kit
ALB / Serum Albumin
Colorimetric (620 nm Absorbance)
250tst  $310.00
Colorimetry Image
Colorimetry Image

BCP Albumin Assay Kit (Colorimetric) - LS-K203
Quantitative Assay Kit
ALB / Serum Albumin
Colorimetric (610 nm Absorbance)
250tst  $310.00
Colorimetry Image
Colorimetry Image

Albumin (Albuminuria) Assay Kit (Fluorometric) - LS-K96
Quantitative Assay Kit
ALB / Serum Albumin
Fluorometric (Ex/Em 600/630 nm)
100assy  $525.00
(a) Relative fluorescence of selected metabolites and proteins found in human urine.  Columns: 1) Human Serum Albumin (30 µg) was assayed and compared with 2) Ascorbic Acid (20 mM), 3) Glucose (20 mM), 4) Creatinine (20 mM), and 5) Urea (20 mM), 6) Lysozyme (30 µg), 7) Trypsin (30 µg), and 8) IgG (30 µg).    (b) Measurement of Albumin in urine from healthy and diabetic donors (40 µl each), and saliva (30 µl). Samples were assayed following kit protocol. Albumin concentrations (in mg/L) are: healthy donor urine: 19.4; diabetic donor urine: 49.0; Saliva: 290.
(a) Relative fluorescence of selected metabolites and proteins found in human urine.  Columns: 1) Human Serum Albumin (30 µg) was assayed and compared with 2) Ascorbic Acid (20 mM), 3) Glucose (20 mM), 4) Creatinine (20 mM), and 5) Urea (20 mM), 6) Lysozyme (30 µg), 7) Trypsin (30 µg), and 8) IgG (30 µg).    (b) Measurement of Albumin in urine from healthy and diabetic donors (40 µl each), and saliva (30 µl). Samples were assayed following kit protocol. Albumin concentrations (in mg/L) are: healthy donor urine: 19.4; diabetic donor urine: 49.0; Saliva: 290.
Showing 1-25 of 887 results

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