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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
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IL27

interleukin 27

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Gene Name: interleukin 27
Family/Subfamily: Interleukin
Synonyms: IL27, IL-27, IL-27 p28 subunit, IL30, Interleukin 27, Interleukin-27 subunit alpha, IL27A, Interleukin 30, IL-27-A, IL27p28, IL-27 subunit alpha, IL-27A, IL27-A, p28
Target Sequences: NM_145659 NP_663634.2 Q8NEV9

Publications (2)

1
IL-27 induces the expression of IDO and PD-L1 in human cancer cells. Carbotti G, Barisione G, Airoldi I, Mezzanzanica D, Bagnoli M, Ferrero S, Petretto A, Fabbi M, Ferrini S. Oncotarget. 2015 6:43267-80. (WB; Human) [Full Text Article] [PubMed:26657115] [PMC:PMC4791231] Related Antibodies: LS-B2719.
2
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. Journal of immunology (Baltimore, Md. : 1950). 2014 Mar;192:2634-42. [Full Text Article] [PubMed:24554774] Related Antibodies: LS-B2719.

Viewing 1-25 of 76 items
IL27 Antibody - Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
IL27 Antibody - Anti-IL-27 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
ELISA, ICC, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $545.00
IL27 Antibody - Anti-IL-27 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human IL27
Human
IHC, IHC-P, WB
Unconjugated
100µl   $485.00
IL27 Antibody - Anti-IL-27 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human IL27
Human
IHC, IHC-P, WB
Biotin Conjugated
100µl   1mg/ml   $385.00
IL27 Antibody - Anti-IL-27 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human IL27
Human
IHC, IHC-P, WB
FITC Conjugated
100µl   1mg/ml   $385.00
IL27 Antibody - Detection limit for recombinant GST tagged IL27 is 0.03 ng/ml as a capture antibody.
Select
Mouse Monoclonal [clone 1A9] (IgG1,k) to Human IL27
Human
ELISA, WB
Unconjugated
100µg   0.44mg/ml   $365.00
IL27 Antibody - Immunohistochemistry of paraffin-embedded human colon tissue.
Select
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $295.00; 200µl   $385.00
Select
Rabbit Polyclonal (IgG) to Human IL27
Human
IP, WB
Unconjugated
100µl   $605.00
IL27 Antibody - Western blot of IL-27 in (A) Jurkat and (B) NIH 3T3 cells using antibody at 1 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human IL27
Human
WB
Unconjugated
100µg   200µl   0.5mg/ml   $335.00
Select
Rabbit Polyclonal to Human IL27
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl   $385.00
Select
Rabbit Polyclonal (IgG) to Human IL27
Human
WB
Biotin Conjugated
100µl   $755.00
IL27 Antibody - Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Select
Rat Monoclonal [clone 3H12.F10] (IgG2a,k) to Mouse IL27
Mouse
ELISA, Flo, WB
Unconjugated
25µl   1mg/ml   $235.00
Select
Mouse Monoclonal (IgG1) to Human IL27
Human
ELISA, IHC, WB
Unconjugated
100µg   $375.00
IL27 Antibody - Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 g) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Select
Rat Monoclonal [clone 3H12.F10] (IgG2a,k) to Mouse IL27
Mouse
ELISA, Flo, WB
Unconjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal to Mouse IL27
Mouse
ELISA, IHC, WB
Biotin (NHS-LC) Conjugated, Azide-free
100µg   $375.00
Select
Rabbit Polyclonal (IgG) to Human IL27
Human
ELISA, IHC, WB
Unconjugated
100µg   $375.00
IL27 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
IL27 Antibody - Western Blot of Biotin Conjugated Rabbit anti-IL-27/p28 antibody. Lane 1: Mouse IL-27/p28. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT.
Select
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, IHC, WB
Biotin Conjugated
100µg   1mg/ml   $425.00
IL27 Antibody - Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.
Select
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, IHC, WB
HRP Conjugated
100µg   1mg/ml   $425.00
Select
Mouse Monoclonal to Mouse IL27
Mouse
ELISA, WB
Unconjugated, Azide-free
100µg   $355.00
Select
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Rat, Human
ELISA, IHC
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal to Human IL27
Human
ELISA, IP, WB
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, WB
Unconjugated
200µl   $590.00
IL27 Antibody - Anti-Mouse IL-27/p28 Antibody - Western Blot. Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Select
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, WB
Unconjugated
100µg   1mg/ml   $425.00
IL27 Antibody - Western blot analysis of IL27 using anti-IL27 antibody
Select
Rabbit Polyclonal to Mouse IL27
Mouse
ELISA, WB
Unconjugated
100µg   $325.00

Viewing 1-25 of 76 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).