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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL27

interleukin 27

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Gene Name: interleukin 27
Family/Subfamily: Interleukin
Synonyms: IL27, IL-27, IL-27 p28 subunit, IL30, Interleukin 27, Interleukin-27 subunit alpha, IL27A, Interleukin 30, IL-27-A, IL27p28, IL-27 subunit alpha, IL-27A, IL27-A, p28
Target Sequences: NM_145659 NP_663634.2 Q8NEV9

Publications (2)

1
IL-27 induces the expression of IDO and PD-L1 in human cancer cells. Carbotti G, Barisione G, Airoldi I, Mezzanzanica D, Bagnoli M, Ferrero S, Petretto A, Fabbi M, Ferrini S. Oncotarget. 2015 6:43267-80. (WB; Human) [Full Text Article] [PubMed:26657115] [PMC:PMC4791231] Related Antibodies: LS-B2719.
2
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. Journal of immunology (Baltimore, Md. : 1950). 2014 Mar;192:2634-42. [Full Text Article] [PubMed:24554774] Related Antibodies: LS-B2719.

Filtered by: None
Target
IL27 (75)
Reactivity
Human (36)
Mouse (42)
Rat (12)
Cat (1)
Application
IHC (17)
IHC-P (8)
WB (67)
Flo (2)
ELISA (39)
ICC (1)
IP (2)
Host
rabbit (67)
mouse (5)
rat (2)
sheep (1)
Isotype
IgG (40)
IgG1 (1)
IgG1,k (1)
IgG2a (1)
Clonality
monoclonal mc (6)
polyclonal pc (69)
Clone
1A9 (1)
3H12.F10 (1)
Format
AP Conjugated (3)
APC (2)
APC Conjugated (5)
Azide-free (2)
Biotin (NHS-LC) Conjugated (1)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (6)
HRP Conjugated (7)
PE Conjugated (5)
Unconjugated (37)
Epitope
aa124-150 (8)
aa126-154 (8)
aa29-234 (8)
aa32-234 (8)
C-Terminus (4)
aa26-185 (1)
aa29-243 (1)
aa31-46 (1)
Publications
No (74)
Yes (1)
Showing 1-25 of 75 results

IHC-plus™ IL27 Antibody LS-B2719
Publications (2)
Publications (2)
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 ug/ml.

IHC-plus™ IL27 Antibody LS-B2565
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
ELISA, ICC, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-IL-27 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of IL-27 in EL4 with IL-27 antibody at 10 ug/ml.

IL27 Antibody (clone 1A9) LS-C139389
Mouse Monoclonal [clone 1A9] (IgG1,k) to Human IL27
Human
ELISA, WB
Unconjugated
100µg 0.44mg/ml  $365.00
Detection limit for recombinant GST tagged IL27 is 0.03 ng/ml as a capture antibody.

IHC-plus™ IL27 Antibody (C-Terminus) LS-B8257
Rabbit Polyclonal to Human IL27
Human
IHC, IHC-P, WB
Unconjugated
100µl  $485.00
Anti-IL-27 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL27 / IL-27 antibody Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IL27 Antibody (C-Terminus, Biotin) LS-C467875
Rabbit Polyclonal to Human IL27
Human
IHC, IHC-P, WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Anti-IL-27 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL27 / IL-27 antibody Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IL27 Antibody LS-C334096
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human colon tissue.
Western blot analysis of extracts of various cell lines.

IL27 Antibody LS-C310763
Rabbit Polyclonal (IgG) to Human IL27
Human
IP, WB
Unconjugated
100µl  $605.00

IL27 Antibody (aa31-46) LS-C726
Rabbit Polyclonal (IgG) to Human IL27
Human
WB
Unconjugated
100µg 200µl 0.5mg/ml  $335.00
Western blot of IL-27 in (A) Jurkat and (B) NIH 3T3 cells using antibody at 1 ug/ml.

IL27 Antibody LS-C341644
Rabbit Polyclonal to Human IL27
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $385.00

IL27 Antibody (Biotin) LS-C522584
Rabbit Polyclonal (IgG) to Human IL27
Human
WB
Biotin Conjugated
100µl  $755.00

IL27 Antibody LS-C666408
Mouse Monoclonal (IgG1) to Human IL27
Human
ELISA, IHC, WB
Unconjugated
100µg  $375.00

IL27 Antibody LS-C745267
Rat Monoclonal (IgG2a) to Mouse IL27
Mouse
ELISA, Flo, WB
Unconjugated
25µl 1mg/ml  $235.00
Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

IL27 Antibody (clone 3H12.F10) LS-C153844
Rat Monoclonal [clone 3H12.F10] (IgG) to Mouse IL27
Mouse
ELISA, Flo, WB
Unconjugated
100µg 1mg/ml  $425.00
Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 g) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.

Mouse Anti-IL27 Secondary Antibody (Azide-free, Biotin (NHS-LC)) LS-C758256
Mouse Monoclonal to Mouse IL27
Mouse
ELISA, IHC, WB
Biotin (NHS-LC) Conjugated, Azide-free
100µg  $365.00

IL27 Antibody LS-C666407
Rabbit Polyclonal (IgG) to Human IL27
Human
ELISA, IHC, WB
Unconjugated
100µg  $375.00

IL27 Antibody (HRP) LS-C154140
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, IHC, WB
HRP Conjugated
100µg 1mg/ml  $425.00

IL27 Antibody (Biotin) LS-C154146
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, IHC, WB
Biotin Conjugated
100µg 1mg/ml  $425.00

IL27 Antibody LS-C809649
Rabbit Polyclonal (IgG) to Human IL27
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Western blot Image

Mouse Anti-IL27 Secondary Antibody (Azide-free) LS-C758255
Mouse Monoclonal to Mouse IL27
Mouse
ELISA, WB
Unconjugated, Azide-free
100µg  $345.00

IL27 Antibody (aa124-150) LS-C329678
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, WB
Unconjugated
200µl  $590.00

IL27 Antibody LS-C487562
Rabbit Polyclonal to Human IL27
Human
ELISA, IP, WB
Unconjugated
50µl  $255.00; 100µl  $305.00; 200µl  $355.00

IL27 Antibody LS-C154133
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
ELISA, WB
Unconjugated
100µg 1mg/ml  $425.00
Anti-Mouse IL-27/p28 Antibody - Western Blot. Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

IL27 Antibody LS-C404073
Rabbit Polyclonal (IgG) to Human IL27
Human
ELISA, IHC
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human cervical cancer using IL27 Polyclonal Antibody at dilution of 1:15.
Immunohistochemistry of paraffin-embedded Human cervical cancer using IL27 Polyclonal Antibody at dilution of 1:15.

IL27 Antibody LS-C400816
Rabbit Polyclonal (IgG) to Human IL27
Human
ELISA, IHC
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human liver cancer using IL27 Polyclonal Antibody at dilution of 1:10.
Immunohistochemistry of paraffin-embedded Human liver cancer using IL27 Polyclonal Antibody at dilution of 1:10.

IL27 Antibody LS-C662931
Rabbit Polyclonal to Mouse IL27
Mouse
ELISA, WB
Unconjugated
100µg  $325.00
Western blot analysis of IL27 using anti-IL27 antibody
Showing 1-25 of 75 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).