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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL27 Antibody (clone 3H12.F10) LS‑C153844

Catalog Number Size Price
LS-C153844-100 100 µg (1 mg/ml) $425 
Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 g) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 g) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
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IL27 Antibody (clone 3H12.F10) LS‑C153844

Antibody:
Rat Monoclonal to Mouse IL27
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153844-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse IL27
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL27 antibody LS-C153844 is an unconjugated rat monoclonal antibody to mouse IL27. Validated for ELISA, Flow and WB.
Target
Mouse IL27
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
3H12.F10
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA
Immunogen
This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E. coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
Usage
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL27
Q8NEV9 NM_145659 NP_663634.2

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Requested From: United States
Date Requested: 8/20/2019
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