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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IL27 Antibody (Biotin) LS‑C154146

Catalog Number Size Price
LS-C154146-100 100 µg (1 mg/ml) $425 
Antibody

IL27 Antibody (Biotin) LS‑C154146

Antibody:
Rabbit Polyclonal to Mouse IL27
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$425 
LS-C154146-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse IL27
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified

Specifications

Description
IL27 antibody LS-C154146 is a biotin-conjugated rabbit polyclonal antibody to mouse IL27. Validated for ELISA, IHC and WB.
Target
Mouse IL27
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
Specificity
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
Usage
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kD in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL27
Q8NEV9 NM_145659 NP_663634.2

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Requested From: United States
Date Requested: 8/25/2019
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