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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B2719-0.05 0.05 mg (1 mg/ml) $425 
IL27 Antibody - Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL27 Antibody - Western blot of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 ug/ml.
IL27 Antibody - Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL27 Antibody - Western blot of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 ug/ml.
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IHCPlus™ Polyclonal Rabbit anti‑Human IL27 Antibody (IHC, WB) LS‑B2719

IHCPlus™ Polyclonal Rabbit anti‑Human IL27 Antibody (IHC, WB) LS‑B2719

Note: This antibody replaces LS-C19596
Antibody:
Rabbit Polyclonal to Human IL27
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2719-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human IL27
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL27 antibody LS-B2719 is an unconjugated rabbit polyclonal antibody to IL27 from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human IL27
Synonyms
IL27 | IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 - 4 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 14 AA synthetic peptide near the center of human IL-27. The immunogen is located within aa 80-130 of IL-27.
Blocking Peptide
LS-E30234 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B2719. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL27
Q8NEV9 NM_145659 NP_663634.2

Validation

IL27 Antibody - Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-27 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ IL27 Antibody for IHC, WB/Western, ELISA LS-B2719 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I. Journal of immunology (Baltimore, Md. : 1950). 2014 Mar;192:2634-42.

IL-27 induces the expression of IDO and PD-L1 in human cancer cells. Carbotti G, Barisione G, Airoldi I, Mezzanzanica D, Bagnoli M, Ferrero S, Petretto A, Fabbi M, Ferrini S. Oncotarget. 2015 6:43267-80. (WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 7/6/2020