(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kD in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitute in deionized water
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.