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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154140-100 100 µg (1 mg/ml) $425 
IL27 Antibody - Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.

Polyclonal Rabbit anti‑Mouse IL27 Antibody (HRP, IHC, WB) LS‑C154140

Polyclonal Rabbit anti‑Mouse IL27 Antibody (HRP, IHC, WB) LS‑C154140

Antibody:
Rabbit Polyclonal to Mouse IL27
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154140-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse IL27
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
IL27 antibody LS-C154140 is an HRP-conjugated rabbit polyclonal antibody to mouse IL27. Validated for ELISA, IHC and WB.
Target
Mouse IL27
Synonyms
IL27 | IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
Specificity
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
Usage
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kD in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL27
Q8NEV9 NM_145659 NP_663634.2

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Requested From: United States
Date Requested: 6/4/2020