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Catalog Number Size Price
LS-C745267-25 25 µl (1 mg/ml) $275 
IL27 Antibody - Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Monoclonal Rat anti‑Mouse IL27 Antibody (clone 3H12.F10, WB) LS‑C745267

Monoclonal Rat anti‑Mouse IL27 Antibody (clone 3H12.F10, WB) LS‑C745267

Antibody:
IL27 Rat anti-Mouse Monoclonal (3H12.F10) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745267-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL27 Rat anti-Mouse Monoclonal (3H12.F10) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL27 antibody LS-C745267 is an unconjugated rat monoclonal antibody to mouse IL27. Validated for ELISA, Flow and WB.
Target
Mouse IL27
Synonyms
IL27 | IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
3H12.F10
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse. Cross-reactivity with IL-27 from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1 µg/ml)
  • ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL27
Q8NEV9 NM_145659 NP_663634.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021