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Catalog Number Size Price
LS-C761813-100 100 µg (1 mg/ml) $574 
TP53 / p53 Antibody - IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
TP53 / p53 Antibody - Western blot testing of human A431 cell lysate with p53 antibody (clone Pab 1801). Expected molecular weight ~53 kDa.
TP53 / p53 Antibody - IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
TP53 / p53 Antibody - Western blot testing of human A431 cell lysate with p53 antibody (clone Pab 1801). Expected molecular weight ~53 kDa.
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Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone PAb 1801, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761813

Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone PAb 1801, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C761813

Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (PAb 1801) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$574
LS-C761813-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (PAb 1801) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
P53 antibody LS-C761813 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for Flow, IF, IHC and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k Monoclonal
Clone
PAb 1801
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.
Immunogen
Human p53 beta-galactosidase fusion protein was used as the immunogen for this antibody. Its epitope maps near the N-Terminus end (aa 32-79) of p53.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis. This antibody binds to an N-terminal portion of both the mutant and wild type p53.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT
Presentation
1X PBS
Storage
Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2024