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Catalog Number Size Price
LS-C761811-20 20 µg (0.2 mg/ml) $371 
LS-C761811-100 100 µg (0.2 mg/ml) $574 
TP53 / p53 Mutant Antibody - IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
TP53 / p53 Mutant Antibody - Western blot testing of human A431 cell lysate with p53 antibody (clone Pab 1801). Expected molecular weight ~53 kDa.
TP53 / p53 Mutant Antibody - IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
TP53 / p53 Mutant Antibody - Western blot testing of human A431 cell lysate with p53 antibody (clone Pab 1801). Expected molecular weight ~53 kDa.
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Monoclonal Mouse anti‑Human TP53 / p53 Mutant Antibody (clone PAb 1801, aa32‑79, IHC, IF, WB) LS‑C761811

Monoclonal Mouse anti‑Human TP53 / p53 Mutant Antibody (clone PAb 1801, aa32‑79, IHC, IF, WB) LS‑C761811

Antibody:
TP53 / p53 Mutant Mouse anti-Human Monoclonal (aa32-79) (PAb 1801) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$371
LS-C761811-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Mutant Mouse anti-Human Monoclonal (aa32-79) (PAb 1801) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 Mutant antibody LS-C761811 is an unconjugated mouse monoclonal antibody to human p53 Mutant (TP53) (aa32-79). Validated for Flow, IF, IHC and WB.
Target
Human TP53 / p53 Mutant
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k Monoclonal
Clone
PAb 1801
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.
Immunogen
Human p53 beta-galactosidase fusion protein was used as the immunogen for this antibody. Its epitope maps near the N-Terminus end (aa 32-79) of p53.
Epitope
aa32-79
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024