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Catalog Number Size Price
LS-B431-50 50 µg (1 mg/ml) $485 
TP53 / p53 Antibody - Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TP53 / p53 Antibody - p53 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells using anti-p53. Protein A purified Mab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overnight at 4° C. After washes cells were incubated with a 1:500 dilution of Alexa Fluor594 Goat-a-Mouse IgG diluted in normal goat serum for 1 h at room temperature. Personnel Communication. Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - Immunofluorescence microscopy of HeLa cells using anti-p53 a 1:100 dilution.  Personnel Communication.  Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - p53 Antibody - Western Blot. Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ug) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Protein A purified Mab anti-p53 overnight at 4° C diluted 1:1500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2000 dilution of HRP Goat-a-Mouse IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - Western blotting using anti-p53.   HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 mg) incubated overnight with anti-p53 overnight at 4° C diluted 1:1,500.  Personnel Communication.  Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TP53 / p53 Antibody - p53 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells using anti-p53. Protein A purified Mab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overnight at 4° C. After washes cells were incubated with a 1:500 dilution of Alexa Fluor594 Goat-a-Mouse IgG diluted in normal goat serum for 1 h at room temperature. Personnel Communication. Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - Immunofluorescence microscopy of HeLa cells using anti-p53 a 1:100 dilution.  Personnel Communication.  Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - p53 Antibody - Western Blot. Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ug) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Protein A purified Mab anti-p53 overnight at 4° C diluted 1:1500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2000 dilution of HRP Goat-a-Mouse IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.
TP53 / p53 Antibody - Western blotting using anti-p53.   HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 mg) incubated overnight with anti-p53 overnight at 4° C diluted 1:1,500.  Personnel Communication.  Kuldeep Patel, Loyola University.
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IHC‑plus™ Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone BP53‑12, IHC, IF, WB) LS‑B431

IHC‑plus™ Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone BP53‑12, IHC, IF, WB) LS‑B431

Note: This antibody replaces LS-C19012
Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (BP53-12) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B431-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (BP53-12) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-B431 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for ChrIP, ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
BP53-12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
Specificity
Reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2000 - 1:10000)
  • Chromatin Immunoprecipitation (1 µg/µl)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B431 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

Validation

TP53 / p53 Antibody - Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TP53 / p53 Antibody (clone BP53-12) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B431 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Cancer. p53, guardian of the genome. Lane DP. Nature. 1992 358:15-6.

p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B, Harris CC. Science (New York, N.Y.). 1991 253:49-53.

Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. Gurney EG, Harrison RO, Fenno J. Journal of virology. 1980 34:752-63.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022