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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C11558-0.1 0.1 mg (1 mg/ml) $395 
Antibody

Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑11, aa181‑190, IHC, WB) LS‑C11558

Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑11, aa181‑190, IHC, WB) LS‑C11558

Antibody:
Mouse Monoclonal to Human TP53 / p53
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$395 
LS-C11558-0.1
0.1 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human TP53 / p53
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa181-190). Validated for IHC, IP and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
DO-11
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000)
  • IHC - Frozen
  • Western blot (1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human p53 (AA 181-190).
Epitope
aa181-190
Specificity
Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.
Usage
Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Requested From: United States
Date Requested: 7/5/2020