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Catalog Number Size Price
LS-C11558-0.1 0.1 mg (1 mg/ml) $422 
Antibody

Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑11, aa181‑190, IHC, WB) LS‑C11558

Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑11, aa181‑190, IHC, WB) LS‑C11558

Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (aa181-190) (DO-11) Antibody
Application:
IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$422
LS-C11558-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (aa181-190) (DO-11) Antibody
Application:
IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa181-190). Validated for IHC, IP and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
DO-11
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Recombinant human p53 (AA 181-190).
Epitope
aa181-190
Specificity
Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.
Applications
  • IHC - Paraffin (1:1000)
  • IHC - Frozen
  • Western blot (1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/29/2022