Target
Rat Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Biotin.
Also available
Unconjugated.
Purification
Protein A purified
Immunogen
Recombinant rat leptin.
Specificity
Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
Applications
- Western blot (1:500 - 1:5000)
- ELISA (1:500 - 1:5000)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA - Direct and indirect: 1:500-1:5000. Western Blot - 1:500-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee