LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Protein A purified
- Western blot (1:5000 - 1:10000)
- ELISA (1:5000 - 1:10000)
Recombinant rat leptin.
Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
ELISA - Direct and indirect: 1:5000-1:10000. Western Blot - 1:5000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
PBS, pH 7.4, no preservatives.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.