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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B1459-50 50 µl (85 mg/ml) $425 
Leptin Antibody - Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ Polyclonal Leptin Antibody (IHC, WB) LS‑B1459

IHCPlus™ Polyclonal Leptin Antibody (IHC, WB) LS‑B1459

Note: This antibody replaces LS-C19017
Antibody:
Rabbit Polyclonal to Mouse Leptin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1459-50
50 µl
85 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse Leptin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Leptin antibody LS-B1459 is an unconjugated rabbit polyclonal antibody to Leptin from human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Mouse Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Specificity
The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Usage
Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Leptin
P41159 NM_000230 NP_000221.1

Validation

Leptin Antibody - Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ Leptin Antibody for IHC, WB/Western, ELISA LS-B1459 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Hypoplastic left heart syndrome is associated with structural and vascular placental abnormalities and leptin dysregulation. Jones HN, Olbrych SK, Smith KL, Cnota JF, Habli M, Ramos-Gonzales O, Owens KJ, Hinton AC, Polzin WJ, Muglia LJ, Hinton RB. Placenta. 2015 36:1078-86. (WB; Mouse)

Customer Reviews (0)


Requested From: United States
Date Requested: 4/1/2020