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IHCPlus™ Leptin Antibody Monoclonal ELISA,IHC,WB LS‑B8192

Catalog Number Size Price
LS-B8192-500 500 µg (1 mg/ml) $425 
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Anti-Human Leptin Western Blot Unreduced
Anti-Human Leptin Western Blot Reduced
Anti-Human Leptin Sandwich ELISA
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Anti-Human Leptin Western Blot Unreduced
Anti-Human Leptin Western Blot Reduced
Anti-Human Leptin Sandwich ELISA
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IHCPlus™ Leptin Antibody Monoclonal ELISA,IHC,WB LS‑B8192

Note: This antibody replaces LS-C104300, LS-C54171, LS-C89454, LS-C104734, LS-C25159
Antibody:
Mouse Monoclonal to Human Leptin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8192-500
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Leptin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Leptin antibody LS-B8192 is an unconjugated mouse monoclonal antibody to human Leptin. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Leptin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 0.4 µg/ml)
  • ELISA (12 - 16 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E.coli derived recombinant Human Leptin.
Specificity
Human Leptin
Usage
Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Leptin
P41159 NM_000230 NP_000221.1

Validation

Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
IHC Validated
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 9/20/2019