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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B7727-50 50 µl (1 mg/ml) $425 
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of extracts from 293 cells, treated with Etoposide 25 uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of extracts from 293 cells, treated with Etoposide 25 uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.
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PathPlus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (aa266‑315, IHC, WB) LS‑B7727

PathPlus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (aa266‑315, IHC, WB) LS‑B7727

Note: This antibody replaces LS-C121035
Antibody:
Rabbit Polyclonal to Human CASP9 / Caspase 9
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7727-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human CASP9 / Caspase 9
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CASP9 (Caspase 9) is a cysteine-aspartic acid protease involved in the execution-phase of cell apoptosis. CASP9 plays a central role in apoptosis and is considered a tumor suppressor. It is important for development of the central nervous system, and absence or mutation of CASP9 leads to abnormal development including neural tube defects, abnormal function and premature death. CASP9 is implicated in the progression of various neurodegenerative diseases including Alzheimer’s, Huntington’s, Parkinson’s and amyotrophic lateral sclerosis (ALS). It can be upregulated in the brain of Alzheimer’s patients. In immunohistochemistry, CASP9 has highest cytoplasmic and nuclear positivity in the brain and heart, medium expression in the liver, pancreas and skeletal muscle, and lowered positivity in all other tissues. References: The International Journal of Biochemistry & Cell Biology. 32 (2): 121–4, PMID: 10687948; Circulation Research. 99 (4): 415–23, PMID: 16857965; Journal of Neuroscience. 2010. 30 (7) 2623-2635; DOI: JNEUROSCI.5115-09.2010; CNS Neurosci Ther. 2018 May;24(5):394-403, PMID: 29365368
Target
Human CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human Caspase 9. (AA Range: 266-315)
Epitope
aa266-315
Specificity
Caspase 9 (Cleaved-Asp315) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315.
Usage
Western Blot: Predicted MW 37 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP9 / Caspase 9
P55211 NM_001229 NP_001220.2

Validation

CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

PathPlus™ CASP9 / Caspase 9 Antibody (aa266-315) for IHC, WB/Western LS-B7727 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 6/4/2020