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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B2021-100 100 µl (0.5 mg/ml) $425 
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of 20 ug of whole cell lysate from HeLa cells with antibody at 1 ug/ml dilution.
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of 20 ug of whole cell lysate from HeLa cells with antibody at 1 ug/ml dilution.
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IHCPlus™ Polyclonal Rabbit anti‑Rat CASP9 / Caspase 9 Antibody (aa345‑359, IHC, WB) LS‑B2021

IHCPlus™ Polyclonal Rabbit anti‑Rat CASP9 / Caspase 9 Antibody (aa345‑359, IHC, WB) LS‑B2021

Note: This antibody replaces LS-C647
Antibody:
Rabbit Polyclonal to Rat CASP9 / Caspase 9
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2021-100
100 µl
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Rat CASP9 / Caspase 9
Application:
IHC, IHC-P, WB
Reactivity:
Rat, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 9 antibody LS-B2021 is an unconjugated rabbit polyclonal antibody to Caspase 9 (CASP9) (aa345-359) from rat. It is reactive with human and rat. Validated for IHC and WB.
Target
Rat CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Rat, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200) & (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A mixture of synthetic peptides corresponding to amino acids 345-359 (CLFPSPPQSLTASMG) and 366-383 (GTRHWYMYSRTSTSPPKA) of rat Caspase-9 (CTD). Percent identity by BLAST analysis: Rat (100%).
Epitope
aa345-359
Specificity
A 45 kD band is detected.
Usage
Immunohistochemistry: LS-B2021 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B2021 was determined to be 1:200.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP9 / Caspase 9
P55211 NM_001229 NP_001220.2

Validation

CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CASP9 / Caspase 9 Antibody (aa345-359) for IHC, WB/Western LS-B2021 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/6/2020