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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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CASP9 / Caspase 9

caspase 9, apoptosis-related cysteine peptidase

CASP9 / Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Gene Name: caspase 9, apoptosis-related cysteine peptidase
Family/Subfamily: Protease , Cysteine C14
Synonyms: CASP9, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, APAF-3, Apoptotic protease Mch-6, CASP-9, Caspase 9, Caspase-9, PPP1R56
Target Sequences: NM_001229 NP_001220.2 P55211

Publications (1)

1
Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis. Martin AG, Fearnhead HO. The Journal of biological chemistry. 2002 277:50834-41. [PubMed:12393884]

Viewing 1-25 of 186 items
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Select
Neuroscience
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $425.00
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Mouse Monoclonal [clone 2-22] (IgG1) to Human CASP9 / Caspase 9
Human, Monkey
IHC, IHC-P, IP, WB
Unconjugated
50µg   1mg/ml   $425.00
CASP9 / Caspase 9 Antibody - Formalin-fixed, paraffin-embedded dog brain sections stained for cleaved Caspase-9 expression using Polyclonal Antibody to (Active) Cleaved Caspase-9 antibody at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-Eosin counterstain.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Dog, Rat, Gerbil, Human
IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
50µl   $335.00
CASP9 / Caspase 9 Antibody - IHC of Caspase-9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections using Polyclonal Antibody to Caspase-9 at 1:2000. Hematoxylin-Eosin counterstain. A: Brain striatum. B: Brain stem motor neuron.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Dog, Rat, Human
IHC, IHC-Fr, IHC-P, IP, WB
Unconjugated
50µl   $335.00
CASP9 / Caspase 9 Antibody - Western blot of Caspase 9 antibody.
Select
Rabbit Polyclonal to Mouse CASP9 / Caspase 9
Mouse, Rat, Human
WB
Unconjugated
30µg   $245.00; 100µg   500µl   $385.00
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Rat CASP9 / Caspase 9
Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $425.00
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
ELISA, ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
0.05mg   1mg/ml   $425.00
CASP9 / Caspase 9 Antibody - Immunohistochemical analysis of Caspase 9 p35 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Select
Mouse Monoclonal [clone LAP6 96-2-22] (IgG1,k) to Human CASP9 / Caspase 9
Human
IHC, IHC-P, WB
Unconjugated
50µl   $425.00
CASP9 / Caspase 9 Antibody - Western blot of Caspase 9 antibody.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Rabbit, Mouse, Bovine, Rat, Hamster, Human
WB
Unconjugated
30µg   $245.00; 100µg   200µl   $395.00
CASP9 / Caspase 9 Antibody - Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Mouse Monoclonal to Human CASP9 / Caspase 9
Mouse, Rat, Human
ICC, IF, IP, WB
Unconjugated
100µl   1mg/ml   $275.00
Select
Rabbit Monoclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Human
ICC, IF, IHC, IP, WB
Unconjugated
50µl   $295.00
CASP9 / Caspase 9 Antibody - Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 Antibody. The picture on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
CASP9 / Caspase 9 Antibody - Western blot of Cleaved-Caspase-9 p35 (D315) antibody
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Sheep, Bovine, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
0.1ml   1mg/ml   $265.00; 0.5ml   $555.00; 1ml   1mg/ml   $925.00
CASP9 / Caspase 9 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00
CASP9 / Caspase 9 Antibody
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Mouse Monoclonal (IgG1,k) to Human CASP9 / Caspase 9
Human
WB
Unconjugated
200µl   $530.00
CASP9 / Caspase 9 Antibody - Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
CASP9 / Caspase 9 Antibody - Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
CASP9 / Caspase 9 Antibody - Immunohistochemical analysis of Caspase 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
CASP9 / Caspase 9 Antibody - Immunohistochemical analysis of Caspase 9 p35 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100µg   $405.00
CASP9 / Caspase 9 Antibody
Select
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
WB
Unconjugated
100µg   500µl   $545.00

Viewing 1-25 of 186 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).