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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B3741-0.05 0.05 mg (1 mg/ml) $425 
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Immunocytochemical staining of K562 cells using Caspase-9 antibody at 2 µg/ml.
CASP9 / Caspase 9 Antibody - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution.
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CASP9 / Caspase 9 Antibody - Immunocytochemical staining of K562 cells using Caspase-9 antibody at 2 µg/ml.
CASP9 / Caspase 9 Antibody - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/ml.
CASP9 / Caspase 9 Antibody - Western blot of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution.
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IHCPlus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (IHC, IF, WB) LS‑B3741

IHCPlus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (IHC, IF, WB) LS‑B3741

Note: This antibody replaces LS-C295
Antibody:
Rabbit Polyclonal to Human CASP9 / Caspase 9
Application:
IHC, IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3741-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human CASP9 / Caspase 9
Application:
IHC, IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 9 antibody LS-B3741 is an unconjugated rabbit polyclonal antibody to human Caspase 9 (CASP9). Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 20 AA peptide near the center of human Caspase-9. The immunogen is located within aa 290-340 of Caspase-9.
Blocking Peptide
LS-E29879 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B3741. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
Usage
Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 46 kDa Observed: 45 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP9 / Caspase 9
P55211 NM_001229 NP_001220.2

Validation

CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

CASP9 / Caspase 9 Antibody - Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CASP9 / Caspase 9 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B3741 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/14/2020