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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP9 / Caspase 9

caspase 9, apoptosis-related cysteine peptidase

CASP9 / Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Gene Name: caspase 9, apoptosis-related cysteine peptidase
Family/Subfamily: Protease , Cysteine C14
Synonyms: CASP9, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, APAF-3, Apoptotic protease Mch-6, CASP-9, Caspase 9, Caspase-9, PPP1R56
Target Sequences: NM_001229 NP_001220.2 P55211

Publications (1)

1
Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis. Martin AG, Fearnhead HO. The Journal of biological chemistry. 2002 277:50834-41. [PubMed:12393884]

Filtered By: None
Target
CASP9 / Caspase 9 (186)
Reactivity
Human (161)
Mouse (61)
Rat (64)
Bovine (19)
Dog (5)
Gerbil (4)
Hamster (1)
Monkey (5)
Rabbit (1)
Sheep (10)
Application
IHC (104)
IHC-Fr (6)
IHC-P (32)
WB (181)
Flo (1)
ELISA (37)
ICC (15)
IF (26)
IP (33)
Peptide-ELISA (11)
Host
rabbit (165)
mouse (21)
Isotype
IgG (126)
IgG1 (5)
IgG1,k (3)
IgG2a (1)
Clonality
monoclonal mc (22)
polyclonal pc (164)
Clone
2-22 (1)
3-20 (1)
32051 (1)
3B8-4G2 (1)
5B4 (1)
LAP6 96-2-22 (1)
Format
AP Conjugated (4)
APC (5)
APC Conjugated (9)
Azide-free (2)
Biotin Conjugated (14)
Cy3 Conjugated (5)
Cy7 Conjugated (5)
FITC Conjugated (13)
HRP Conjugated (10)
PE Conjugated (9)
Unconjugated (117)
Epitope
aa1-200 (20)
aa331-416 (10)
Internal (9)
pSer196 (9)
aa1-134 (8)
aa331-411 (8)
N-Terminus (4)
pThr125 (3)
140-220 aa (2)
60-140 aa (2)
80-160 aa (2)
90-170 aa (2)
Asp315 (2)
Glu119 (2)
aa188-206 (2)
aa299-318 (2)
pTyr153 (2)
240-320 aa, Internal (1)
250-330 aa, C-terminal (1)
280-360 aa, C-terminal (1)
Ala149 (1)
Arg192 (1)
Asp330 (1)
Asp353 (1)
C-Terminus (1)
Cleave Site (1)
Gly140 (1)
Tyr153 (1)
aa110-159 (1)
aa112-130 (1)
aa119-168 (1)
aa162-211 (1)
aa183-198 (1)
aa183-211 (1)
aa266-315 (1)
aa281-330 (1)
aa3-228 (1)
aa301-305 (1)
aa323-372 (1)
aa345-359 (1)
aa41-56 (1)
aa88-416 (1)
aa91-140 (1)
pSer144 (1)
Publications
No (186)
Showing 1-25 of 186 results

CASP9 / Caspase 9 Antibody (clone 2-22) IHC-plus™ LS-B3672
Mouse Monoclonal [clone 2-22] (IgG1) to Human CASP9 / Caspase 9
Human, Monkey
IHC IHC-P IP WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

CASP9 / Caspase 9 Antibody LS-C148247
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Dog, Rat, Gerbil, Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Formalin-fixed, paraffin-embedded dog brain sections stained for cleaved Caspase-9 expression using Polyclonal Antibody to (Active) Cleaved Caspase-9 antibody at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human brain sections stained for cleaved Caspase-9 expression using Polyclonal Antibody to (Active) Cleaved Caspase-9 antibody at 1:2000. A. Section from a patient 24 hr after head trauma. B. Control: section from a patient with no known neurological disease or head injury. Hematoxylin-Eos in counterstain.

CASP9 / Caspase 9 Antibody LS-C48207
Rabbit Polyclonal to Mouse CASP9 / Caspase 9
Mouse, Rat, Human
WB
Unconjugated
30µg  $245.00; 100µg 500µl  $415.00
Western blot of Caspase 9 antibody.

CASP9 / Caspase 9 Antibody IHC-plus™ LS-B3741
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
ELISA ICC IF IHC IHC-P IP WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-Caspase 9 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

CASP9 / Caspase 9 Antibody (aa345-359) IHC-plus™ LS-B2021
Rabbit Polyclonal (IgG) to Rat CASP9 / Caspase 9
Rat, Human
IHC IHC-P WB
Unconjugated
100µl 0.5mg/ml  $425.00
Anti-Caspase 9 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of 20 ug of whole cell lysate from HeLa cells with antibody at 1 ug/ml dilution.

CASP9 / Caspase 9 Antibody (clone LAP6 96-2-22) IHC-plus™ LS-B241
Mouse Monoclonal [clone LAP6 96-2-22] (IgG1,k) to Human CASP9 / Caspase 9
Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-Caspase 9 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

CASP9 / Caspase 9 Antibody LS-C747168
Rabbit Monoclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Human
ICC IF IHC IP WB
Unconjugated
50µl  $295.00

CASP9 / Caspase 9 Antibody (aa91-140) LS-C117554
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using Caspase 9 Antibody. The picture on the right is blocked with the synthesized peptide.

CASP9 / Caspase 9 Antibody LS-C148248
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Dog, Rat, Gerbil, Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
IHC of Caspase-9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections using Polyclonal Antibody to Caspase-9 at 1:2000. Hematoxylin-Eosin counterstain. A: Brain striatum. B: Brain stem motor neuron.
Western blot of Caspase-9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 kD pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.

CASP9 / Caspase 9 Antibody (phospho-Ser196) LS-C97424
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-Phospho-Caspase 9-S196 antibody is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.

CASP9 / Caspase 9 Antibody LS-C12569
Mouse Monoclonal (IgG1,k) to Human CASP9 / Caspase 9
Human
WB
Unconjugated
200µl  $530.00

CASP9 / Caspase 9 Antibody (aa266-315) IHC-plus™ LS-B7727
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Caspase 9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Western blot of extracts from 293 cells, treated with Etoposide 25 uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.

CASP9 / Caspase 9 Antibody (Internal) LS-C413408
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of Caspase 9 p35 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CASP9 / Caspase 9 Antibody (Internal) LS-C353929
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Caspase 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CASP9 / Caspase 9 Antibody (Internal) LS-C351940
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CASP9 / Caspase 9 Antibody (Internal) LS-C351948
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Rat, Human
ICC IF IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Caspase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CASP9 / Caspase 9 Antibody LS-C659588
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
ELISA Flo IF IHC IP WB
Unconjugated
100µg  $405.00

CASP9 / Caspase 9 Antibody (N-Terminus) LS-C48213
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
WB
Unconjugated
100µg 500µl  $605.00

CASP9 / Caspase 9 Antibody LS-C347586
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human
WB
Unconjugated
30µg  $245.00; 100µg  $425.00

CASP9 / Caspase 9 Antibody LS-C48128
Rabbit Polyclonal to Human CASP9 / Caspase 9
Rabbit, Mouse, Bovine, Rat, Hamster, Human
WB
Unconjugated
30µg  $245.00; 100µg 200µl  $425.00
Western blot of Caspase 9 antibody.

CASP9 / Caspase 9 Antibody LS-C413507
Mouse Monoclonal to Human CASP9 / Caspase 9
Mouse, Rat, Human
ICC IF IP WB
Unconjugated
100µl 1mg/ml  $275.00
Immunofluorescent analysis of Caspase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Caspase 9 expression in HeLa (A); Jurkat (B); HepG2 (C); mouse heart (D); rat heart (E) whole cell lysates.

CASP9 / Caspase 9 Antibody LS-C417565
Rabbit Polyclonal to Human CASP9 / Caspase 9
Mouse, Rat, Human
ICC IF IHC WB
Unconjugated
100µg 1mg/ml  $305.00
Western blot of Caspase 9 expression in TNF-a treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

CASP9 / Caspase 9 Antibody (aa183-211) LS-C99786
Rabbit Polyclonal to Human CASP9 / Caspase 9
Human
IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of CASP9 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

CASP9 / Caspase 9 Antibody (90-170 aa) LS-C382461
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Human, Monkey
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

CASP9 / Caspase 9 Antibody LS-C496650
Rabbit Polyclonal (IgG) to Human CASP9 / Caspase 9
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded mouse stomach using CASP9 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CASP9 antibodyat dilution of 1:100 (40x lens).
Showing 1-25 of 186 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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