Features: - Blue-gray chromogen precipitant - Precipitated by Peroxidase - Suitable for non-aqueous (permanent) mounting - Sufficient reagents to produce 300 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - Additional substrate properties contrast with tissue pigments - No wait times for mixing and dissolving powders or tablets - One year expiry date - CE Mark Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot Details: Vector® VIP and ImmPACT™ VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector® VIP and ImmPACT™ VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted. Vector® VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® VIP substrate is equivalent to Vector® DAB Substrate. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4° C, this kit is stable for one year.