Features: - 2 to 4 times more sensitive than original substrate kit - Blue-gray chromogen precipitant - Precipitated by Peroxidase - Suitable for aqueous (hardening) and non-aqueous (permanent) mounting - For used on nylon or PVDF membranes but is not recommended for nitrocellulose membranes - Sufficient reagents to produce 120 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - Stock solutions supplied in convenient dropper bottles promoting ease of handling - Working solution stable for up to 14 days at 4 °C - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot Details: Vector® SG and ImmPACT™ SG HRP substrates produce blue-gray reaction products that can be used as a single label or as a second color for multiple antigen labeling. With the aid of imaging systems and software, the spectral profile of both chromogens can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield or electron microscopy. Vector® SG and ImmPACT™ SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted. This product utilizes Vector® Laboratories novel ImmPACT™ technology to generate an intense blue-gray reaction product that is 2-4 times more sensitive than Vector® SG substrate kit (LS-J1082). This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ SG reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 °C.