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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Vector® NovaRED™ Peroxidase (HRP) Substrate Kit - LS-J1084

Available for shipment within the USA only
Catalog
Size
Price
LS-J1084-1
1 ea
$180

Available for shipment within the USA only

Product Description

This substrate produces a red chromogen precipitant in the presence of peroxidase enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, and ISH, as well as various blotting applications. Sufficient to produce 120 ml of working solution.

Specifications

Details
Features: - Red chromogen precipitant - Precipitated by Peroxidase - Suitable for non-aqueous (permanent) mounting - Sufficient reagents to produce 300 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - No wait times for mixing and dissolving powders or tablets - One year expiry date - CE Mark Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot Details: Vector® NovaRED™ and ImmPACT™ NovaRED™ HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield microscopy. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates can be used for both manual and automated staining methods. Vector® NovaRED™ Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® NovaRED™ substrate is equivalent to Vector® DAB Substrate, and 4 times greater than AEC. This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 °C, this kit is stable for one year.
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPRESS™ anti-mouse Ig reagent (LS-J1061), and Vector® NovaRED® peroxidase substrate (LS-J1084; red). Hematoxylin QS counterstain.

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Requested From: United States
Date Requested: 7/15/2019
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