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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Vector Red™ Alkaline Phosphatase (Red AP) Substrate Kit - LS-J1086

Available for shipment within the USA only
Catalog
Size
Price
LS-J1086-1
1 ea
$185

Available for shipment within the USA only

Product Description

This substrate produces a magenta chromogen/red fluorescent precipitant in the presence of Alkaline Phosphatase (AP) enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, IF, and ISH, as well as various blotting applications. Sufficient to produce 200 ml of working solution.

Specifications

Details
Features: - magenta chromogen/red fluorescent precipitant - Fluorescent (Excitation: broad excitation spectrum, approx. 365 nm to 560 nm / Emission: Above 560 nm) - Precipitated by Alkaline Phosphatase (AP) - Suitable for non-aqueous (permanent) mounting - Sufficient reagents to produce 200 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, IF, ISH, Blotting Applications, and Elispot - No wait times for mixing and dissolving powders or tablets - Suitable for use in single or multiple labeling assays - Stock solutions in convenient dropper bottles - Heat Stable - One year expiry date - CE Mark Applications: - Immunohistochemistry - Immunocytochemistry - Immunofluorescence - In situ hybridization - Blotting Applications - Elispot Details: Vector Red™ AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector Red™ substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector Red™ can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. The bright red to orange fluorescence of the Vector Red™ reaction product can be visualized using Daylight® 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunofluorescence

Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).
Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).

Immunohistochemistry

Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunofluorescence

Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).
Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).

Immunohistochemistry

Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunofluorescence

Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).
Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).

Immunohistochemistry

Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunofluorescence

Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).
Small Bowel (frozen, double label): PGP9.5 (m), M.O.M.™ Basic Kit, VECTASTAIN® ABC AP Standard Kit, Vector Red™ substrate (red); Desmin (m), M.O.M.™ Fluorescein Kit (green).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red); CD3 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® DAB substrate (brown).

Immunohistochemistry

Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).
Tumor (double label): p53 protein (m), VECTASTAIN® ABC-AP Kit, Vector Red™ substrate (red); Pan-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® SG Substrate (blue/gray).

Immunohistochemistry

Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Lymph Node (double label): Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ Substrate (red); Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Requested From: United States
Date Requested: 4/19/2019
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