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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Vector® Blue Alkaline Phosphatase (Blue AP) Substrate Kit - LS-J1089

Available for shipment within the USA only
Catalog
Size
Price
LS-J1089-1
1 ea
$185

Available for shipment within the USA only

Product Description

This substrate produces a blue chromogen/blue fluorescent precipitant in the presence of Alkaline Phosphatase (AP) enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, and ISH, as well as various blotting applications. Sufficient to produce 200 ml of working solution.

Specifications

Details
Features: - Blue chromogen /blue fluorescent precipitant - Fluorescent (Excitation: broad, peak at ~500 nm / Emission: broad, peak at ~680 nm) - Precipitated by Alkaline Phosphatase (AP) - Suitable for non-aqueous (permanent) mounting - Sufficient reagents to produce 200 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, IF, ISH, Blotting Applications, and Elispot - No wait times for mixing and dissolving powders or tablets - Suitable for use in single or multiple labeling assays - Heat Stable - Contrast with Tissue Pigments - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - Immunofluorescence - In situ hybridization - Blotting Applications - Elispot Details: The Vector Blue™ AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Vector Blue™ is also fluorescent. Sections stained with the Vector Blue™ substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount) must be used because the reaction product is soluble in xylene. Vector Blue™ can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.
Restrictions
For research use only. Available for shipment within the USA only.

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Images

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Immunohistochemistry

Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Requested From: United States
Date Requested: 7/15/2019
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