Features: - The most sensitive red AP substrate - 2 to 4 times more sensitive than original substrate kit - Magenta chromogen precipitant - Fluorescent (Red)(Excitation: broad excitation spectrum, approx. 365 nm to 560 nm; Emission: Above 560 nm) - Precipitated by Alkaline Phosphatase (AP) - Suitable for aqueous (hardening and non-hardening) and non-aqueous (permanent) mounting - 120 ml Ready-to-Use diluent and stock solutions in dropper bottles - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, IF, ISH, Blotting Applications, and Elispot - Suitable for use in single or multiple labeling assays - Heat Stable - Contrast with Tissue Pigments - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - Immunofluorescence - In situ hybridization - Blotting Applications - Elispot Details: ImmPACT™ Vector Red™ AP Substrate Kit, a new formulation for the Vector Red™ AP substrate, produces a bright red reaction product that is crisper and is 2-4 times more sensitive than the original Vector Red™ substrate kit. This product consists of 120 ml of ready-to-use diluent and concentrated stock solutions in convenient dropper bottles. Both Vector Red™ and ImmPACT™ Vector Red™ alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector Red™ or ImmPACT™ Vector Red™ AP chromogen can be dehydrated, cleared, and permanently mounted. Both chromogen formulations can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of Vector Red™ or ImmPACT™ Vector Red™ substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Both formulations can be used for either manual or automated staining methods. The bright fluorescence of the Vector Red™ or ImmPACT™ Vector Red™ reaction products are visible using DyLight® 594, Texas Red® or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector Red™ and ImmPACT™ Vector Red™ reaction products have a wider dynamic range, offering better quantitation of targets on blots.
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