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| Catalog Number | Size | Price |
|---|---|---|
| LS-C828413-100 | 100 µg (1 mg/ml) | $424 |
Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑1, IHC, IF, WB) LS‑C828413
Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone DO‑1, IHC, IF, WB) LS‑C828413
Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (DO-1) Antibody
Application:
IHC-P, IHC-Fr, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TP53 / p53 Mouse anti-Human Monoclonal (DO-1) Antibody
Application:
IHC-P, IHC-Fr, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
P53 antibody LS-C828413 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for ELISA, Flow, ICC, IF, IHC and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
DO-1
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
DO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.
Specificity
DO-1 recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (amino acids 11-25) of p53.
Applications
- IHC - Paraffin (1:500 - 1:2000)
- IHC - Frozen (1:500 - 1:2000)
- ICC
- Immunofluorescence
- Western blot (1:500 - 1:1000)
- Flow Cytometry (1:500 - 1:1000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C for short term storage. Aliquot and store at -20°C for long term storage. Avoid freeze/thaw cycles. Prior to use, thaw aliquot slowly in the dark at ambient temperature. Spin down and prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Store working dilutions at 4°C, and preferably use the same day. Remove slight precipitation with centrifugation.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TP53 / p53
Publications (0)
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Chromatin Immunoprecipitation
Species:
Mouse, Human, Rat, Bovine, Hamster, Chicken
Applications:
IHC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/3/2024
Date Requested: 5/3/2024
