Human TP53 / p53

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

CTA53-1

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Human recombinant protein.

Human TP53 / p53

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons. [RefSeq] Titering of the p53 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3