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Catalog Number Size Price
LS-C801062-100 100 µl (1 mg/ml) $379 
LS-C801062-200 200 µl (1 mg/ml) $421 
NOS3 / eNOS Antibody - Phospho-eNOS (Ser1177) antibody antibody for IHC in human colon tissue
NOS3 / eNOS Antibody - 1:200 staining mouse intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NOS3 / eNOS Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NOS3 / eNOS Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NOS3 / eNOS Antibody - Western blot analysis of eNOS phosphorylation expression in Insulin treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NOS3 / eNOS Antibody - Western blot analysis of Phospho-eNOS (Ser1177) expression in various lysates
NOS3 / eNOS Antibody - Phospho-eNOS (Ser1177) antibody antibody for IHC in human colon tissue
NOS3 / eNOS Antibody - 1:200 staining mouse intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NOS3 / eNOS Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NOS3 / eNOS Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NOS3 / eNOS Antibody - Western blot analysis of eNOS phosphorylation expression in Insulin treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NOS3 / eNOS Antibody - Western blot analysis of Phospho-eNOS (Ser1177) expression in various lysates
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Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (phospho‑Ser1177, IHC, IF, WB) LS‑C801062

Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (phospho‑Ser1177, IHC, IF, WB) LS‑C801062

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801062-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ENOS antibody LS-C801062 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (pSer1177) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human eNOS around the phosphorylation site of Serine 1177.
Epitope
pSer1177
Specificity
Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Serine 1177.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 3/1/2024