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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NOS3 / eNOS

nitric oxide synthase 3 (endothelial cell)

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gene Name: nitric oxide synthase 3 (endothelial cell)
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3
Target Sequences: NM_000603 NP_000594.2 P29474

Publications (1)

1
Restoration of perivascular adipose tissue function in diet-induced obese mice without changing bodyweight. Xia N, Weisenburger S, Koch E, Burkart M, Reifenberg G, Förstermann U, Li H. British journal of pharmacology. 2017 174:3443-3453. (WB; Mouse) [Full Text Article] [PubMed:28055105] [PMC:PMC5610154] Related Antibodies: LS-C288673.

Filtered by: None
Target
NOS3 / eNOS (206)
Reactivity
Human (152)
Mouse (69)
Rat (77)
Bat (2)
Bovine (51)
Cow (8)
Dog (18)
Guinea pig (5)
Hamster (2)
Horse (3)
Monkey (5)
Pig (23)
Rabbit (17)
Sheep (6)
Application
IHC (24)
IHC-P (14)
WB (194)
Flo (9)
DB (8)
ELISA (69)
ICC (17)
IF (29)
IP (14)
Peptide-ELISA (9)
RIA (1)
Host
rabbit (190)
mouse (16)
Isotype
IgG (99)
IgG1 (4)
IgG1,k (9)
IgG2a,k (1)
Clonality
monoclonal mc (17)
polyclonal pc (189)
Clone
15E2 (1)
1D12 (1)
6G3 (1)
6H2 (3)
Mix (1)
Format
AP Conjugated (7)
APC (7)
APC Conjugated (14)
Azide-free (3)
Biotin Conjugated (17)
Cy3 Conjugated (7)
Cy7 Conjugated (7)
FITC Conjugated (17)
HRP Conjugated (15)
PE Conjugated (14)
Unconjugated (108)
Epitope
pSer1177 (19)
aa522-705 (16)
Ser1177 (8)
aa48-75 (8)
aa519-702 (8)
aa756-888 (8)
aa758-1004 (8)
aa888-1001 (8)
aa106-118 (7)
pSer617 (7)
N-Terminus (6)
pThr494 (4)
pSer615 (3)
1110-1190 aa (2)
430-510 aa (2)
550-630 aa (2)
aa1181-1194 (2)
pThr495 (2)
1120-1200 aa (1)
C-Terminus (1)
Gly488 (1)
Ile611 (1)
Internal (1)
Ser116 (1)
Ser1170 (1)
aa1126-1175 (1)
aa1144-1193 (1)
aa1145-1194 (1)
aa1186-1203 (1)
aa1202-1205 (1)
aa439-488 (1)
aa462-511 (1)
aa575-625 (1)
aa581-630 (1)
aa597-611 (1)
pSer113 (1)
pSer114 (1)
pSer1176 (1)
pSer1179 (1)
Publications
No (205)
Yes (1)
Showing 1-25 of 206 results

IHC-plus™ NOS3 / eNOS Antibody (aa597-611) LS-B6341
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey
ICC, IHC, IHC-P, WB
Unconjugated
50µl  $485.00
Anti-ENOS antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ NOS3 / eNOS Antibody (aa1186-1203) LS-B2095
Rabbit Polyclonal to Human NOS3 / eNOS
Human
IHC, IHC-P, IP, WB
Unconjugated
1ea  $425.00
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC-plus™ NOS3 / eNOS Antibody (aa1181-1194) LS-B5676
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NOS3 / eNOS Antibody (phospho-Ser1177) LS-C352612
Rabbit Polyclonal to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Sheep, Rat, Pig, Human
ICC, IF, IP, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunofluorescent analysis of eNOS (pS1177) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of eNOS (pS1177) expression in A549 (A); HUVEC (B); rat brain (C) whole cell lysates.

NOS3 / eNOS Antibody (aa575-625) LS-C288673
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human
IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00

NOS3 / eNOS Antibody (Ser1177, FITC) LS-C256689
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human
ELISA, Flo, ICC, IF, WB
FITC Conjugated
200µl  $740.00

NOS3 / eNOS Antibody (phospho-Ser1179) LS-C37769
Rabbit Polyclonal (IgG) to Bovine NOS3 / eNOS
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Human
ELISA, IF, WB
Unconjugated
50µg  $605.00

IHC-plus™ NOS3 / eNOS Antibody (clone 6H2) LS-B3902
Mouse Monoclonal [clone 6H2] (IgG1) to Human NOS3 / eNOS
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $425.00
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

NOS3 / eNOS Antibody (aa522-705) LS-C295648
Rabbit Polyclonal to Pig NOS3 / eNOS
Rat, Pig
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $315.00; 1ml 0.5mg/ml  $560.00
Western blot of recombinant NOS3 / eNOS encoding amino acids 522-705.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot; Sample: Rat Heart lysate; Primary Ab: 1µg/ml Rabbit Anti-Porcine NOS3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

NOS3 / eNOS Antibody LS-C23048
Mouse Monoclonal (IgG1,k) to Bovine NOS3 / eNOS
Bovine
ELISA, IP, WB
Unconjugated
100µg  $635.00

NOS3 / eNOS Antibody (clone 1D12) LS-C197792
Mouse Monoclonal [clone 1D12] (IgG2a,k) to Human NOS3 / eNOS
Human
ELISA, WB
Unconjugated
100µg  $365.00
Detection limit for recombinant GST tagged NOS3 is 3 ng/ml as a capture antibody.

NOS3 / eNOS Antibody LS-C37744
Rabbit Monoclonal (IgG) to Human NOS3 / eNOS
Bovine, Rat, Human
IP, WB
Unconjugated
100µl  $530.00

NOS3 / eNOS Antibody (clone 6H2) LS-C359280
Mouse Monoclonal [clone 6H2] (IgG1) to Human NOS3 / eNOS
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $335.00
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.

IHC-plus™ NOS3 / eNOS Antibody LS-B5208
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Dog, Bovine, Rat, Pig, Human
IHC, IHC-P
Unconjugated
250µl  $585.00
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ NOS3 / eNOS Antibody (aa1181-1194) LS-B8720
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-NOS3 / eNOS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB: HUVEC cell lysate was probed with anti-eNOS/NOS III (1 ug/ml).

IHC-plus™ NOS3 / eNOS Antibody LS-B3674
Rabbit Polyclonal to Human NOS3 / eNOS
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $495.00
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

NOS3 / eNOS Antibody LS-C413529
Mouse Monoclonal (IgG1) to Human NOS3 / eNOS
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of eNOS expression in rat heart (A) whole cell lysates.

NOS3 / eNOS Antibody (aa1145-1194) LS-C118001
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549, K562, and COLO205 cells, using eNOS Antibody. The lane on the right is blocked with the synthesized peptide.

NOS3 / eNOS Antibody LS-C355709
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Guinea pig, Sheep, Rat, Horse, Bat, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl  $425.00
IHC using eNOS Antibody
IHC using eNOS Antibody

NOS3 / eNOS Antibody (phospho-Ser1177) LS-C801062
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunohistochemistry Image

NOS3 / eNOS Antibody (aa48-75) LS-C162945
Rabbit Polyclonal to Human NOS3 / eNOS
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of NOS3 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

NOS3 / eNOS Antibody (clone Mix) LS-C387466
Mouse Monoclonal [clone Mix] to Human NOS3 / eNOS
Mouse, Rat, Human
WB
Unconjugated
50µl  $235.00; 100µl  $265.00; 200µl  $335.00
Western blot of eNOS antibody

NOS3 / eNOS Antibody (C-Terminus) LS-C413412
Rabbit Polyclonal to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Sheep, Bovine, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunohistochemical analysis of eNOS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of eNOS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NOS3 / eNOS Antibody (phospho-Ser1177) LS-C23072
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Dog, Bovine, Rat, Human
WB
Unconjugated
100µl  $710.00

NOS3 / eNOS Antibody LS-C800228
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image
Showing 1-25 of 206 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).