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IHCPlus™ NOS3 / eNOS Antibody aa1186‑1203 Polyclonal IHC,IP,WB LS‑B2095

Catalog Number Size Price
LS-B2095-1 1 ea $425 
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHCPlus™ NOS3 / eNOS Antibody aa1186‑1203 Polyclonal IHC,IP,WB LS‑B2095

Note: This antibody replaces LS-C11688
Antibody:
Rabbit Polyclonal to Human NOS3 / eNOS
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2095-1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NOS3 / eNOS
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
eNOS antibody LS-B2095 is an unconjugated rabbit polyclonal antibody to human eNOS (NOS3 ). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human eNOS amino acids 1186-1203 (RGAVPWAFDPPGSDTNSP). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey, Panda (89%); Guinea pig (83%).
Blocking Peptide
LS-E47576 - Liquid - 200 µg - $145.00
Immunizing peptide used to generate LS-B2095. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1186-1203
Specificity
Human eNOS amino acids 1186-1203 (RGAVPWAFDPPGSDTNSP)1,2,3
Usage
Immunohistochemistry: LS-B2095 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2095 was determined to be 1:50.
Presentation
Lyophilized antiserum
Reconstitution
100 ul distilled water containing 0.05% sodium azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

Validation

Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC Validated
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

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Requested From: United States
Date Requested: 9/21/2019