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Catalog Number Size Price
LS-B2095-1 1 Each $485 
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1186‑1203, IHC, WB) LS‑B2095

IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1186‑1203, IHC, WB) LS‑B2095

Note: This antibody replaces LS-C11688
Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1186-1203) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2095-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1186-1203) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ENOS antibody LS-B2095 is an unconjugated rabbit polyclonal antibody to human eNOS (NOS3) (aa1186-1203). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Human eNOS amino acids 1186-1203 (RGAVPWAFDPPGSDTNSP). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey, Panda (89%); Guinea pig (83%).
Epitope
aa1186-1203
Specificity
Human eNOS amino acids 1186-1203 (RGAVPWAFDPPGSDTNSP)1,2,3
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2095 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2095 was determined to be 1:50.
Presentation
Lyophilized antiserum
Reconstitution
100 ul distilled water containing 0.05% sodium azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

Validation

NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ NOS3 / eNOS Antibody (aa1186-1203) for IHC, WB/Western, IP LS-B2095 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/28/2024