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Catalog Number Size Price
LS-B5676-50 50 µg (1 mg/ml) $645 
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1181‑1194, IHC, WB) LS‑B5676

IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1181‑1194, IHC, WB) LS‑B5676

Note: This antibody replaces LS-C37746
Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$645
LS-B5676-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
ENOS antibody LS-B5676 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (aa1181-1194) from human. It is reactive with human, monkey and rabbit. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%); Dog, Bat, Pig (86%).
Epitope
aa1181-1194
Specificity
Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot and IHC-P. Western Blot Analysis: 0.5-2 ug/ml of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
Presentation
Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

Validation

NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ NOS3 / eNOS Antibody (aa1181-1194) for IHC, WB/Western LS-B5676 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2024