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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5676-50 | 50 µg (1 mg/ml) | $645 |
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2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1181‑1194, IHC, WB) LS‑B5676
IHC‑plus™ Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (aa1181‑1194, IHC, WB) LS‑B5676
Note: This antibody replaces LS-C37746
Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NOS3 / eNOS Rabbit anti-Human Polyclonal (aa1181-1194) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
ENOS antibody LS-B5676 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (aa1181-1194) from human. It is reactive with human, monkey and rabbit. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Synonyms
NOS3 | Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3
Host
Rabbit
Reactivity
Human, Monkey, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%); Dog, Bat, Pig (86%).
Epitope
aa1181-1194
Specificity
Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot and IHC-P. Western Blot Analysis: 0.5-2 ug/ml of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
Presentation
Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NOS3 / eNOS
Validation
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NOS3 / eNOS Antibody (aa1181-1194) for IHC, WB/Western LS-B5676 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Bovine, Human, Mouse, Rat, Dog, Guinea pig, Pig
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human
Applications:
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse, Rat, Dog, Pig, Rabbit, Sheep
Applications:
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
