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IHCPlus™ NOS3 / eNOS Antibody aa1181‑1194 Polyclonal IHC,WB LS‑B5676

Catalog Number Size Price
LS-B5676-50 50 µg (1 mg/ml) $425 
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHCPlus™ NOS3 / eNOS Antibody aa1181‑1194 Polyclonal IHC,WB LS‑B5676

Note: This antibody replaces LS-C37746
Antibody:
Rabbit Polyclonal to Human NOS3 / eNOS
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B5676-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NOS3 / eNOS
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
eNOS antibody LS-B5676 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3 ) from human, monkey and rabbit. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NOS3 / eNOS
Host
Rabbit
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%); Dog, Bat, Pig (86%).
Epitope
aa1181-1194
Specificity
Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.
Usage
Suitable for use in Western Blot and IHC-P. Western Blot Analysis: 0.5-2 ug/ml of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
Presentation
Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS3 / eNOS
P29474 NM_000603 NP_000594.2

Validation

Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019