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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NOS3 / eNOS

nitric oxide synthase 3 (endothelial cell)

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Gene Name: nitric oxide synthase 3 (endothelial cell)
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3
Target Sequences: NM_000603 NP_000594.2 P29474

Publications (1)

1
Restoration of perivascular adipose tissue function in diet-induced obese mice without changing bodyweight. Xia N, Weisenburger S, Koch E, Burkart M, Reifenberg G, Förstermann U, Li H. British journal of pharmacology. 2017 174:3443-3453. (WB; Mouse) [Full Text Article] [PubMed:28055105] [PMC:PMC5610154] Related Antibodies: LS-C288673.

Viewing 1-25 of 207 items
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Horse, Bat, Human, Monkey
ICC, IHC, IHC-P, WB
Unconjugated
50µl   $485.00
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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Rabbit Polyclonal to Human NOS3 / eNOS
Human
IHC, IHC-P, IP, WB
Unconjugated
1ea   $425.00
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $425.00
NOS3 / eNOS Antibody - Immunofluorescent analysis of eNOS (pS1177) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Select
Rabbit Polyclonal to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Sheep, Rat, Pig, Human
ICC, IF, IP, WB
Unconjugated
100µl   1mg/ml   $265.00
Select
(1) Publication(s)
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human
IP, WB
Unconjugated
100µl   0.2mg/ml   $395.00
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Human
ELISA, Flo, ICC, IF, WB
FITC Conjugated
200µl   $740.00
Select
Rabbit Polyclonal (IgG) to Bovine NOS3 / eNOS
Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Human
ELISA, IF, WB
Unconjugated
50µg   $605.00
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Select
Mouse Monoclonal [clone 6H2] (IgG1) to Human NOS3 / eNOS
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $425.00
NOS3 / eNOS Antibody - Western blot of recombinant NOS3 / eNOS encoding amino acids 522-705.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Pig NOS3 / eNOS
Rat, Pig
WB
Unconjugated
20µl   0.5mg/ml   $205.00; 100µl   0.5mg/ml   $265.00; 200µl   0.5mg/ml   $315.00; 1ml   0.5mg/ml   $560.00
Select
Mouse Monoclonal (IgG1,k) to Bovine NOS3 / eNOS
Bovine
ELISA, IP, WB
Unconjugated
100µg   $635.00
NOS3 / eNOS Antibody - Detection limit for recombinant GST tagged NOS3 is 3 ng/ml as a capture antibody.
Select
Mouse Monoclonal [clone 1D12] (IgG2a,k) to Human NOS3 / eNOS
Human
ELISA, WB
Unconjugated
100µg   $365.00
Select
Rabbit Monoclonal (IgG) to Human NOS3 / eNOS
Bovine, Rat, Human
IP, WB
Unconjugated
100µl   $530.00
NOS3 / eNOS Antibody - Confocal immunofluorescence of eNos Antibody (S1177) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Select
Rabbit Polyclonal to Human NOS3 / eNOS
Human
Flo, IF, WB
Unconjugated
400µl   $325.00
NOS3 / eNOS Antibody - Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.
Select
Mouse Monoclonal [clone 6H2] (IgG1) to Human NOS3 / eNOS
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $335.00
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Dog, Bovine, Rat, Pig, Human
IHC, IHC-P
Unconjugated
250µl   $585.00
NOS3 / eNOS Antibody - Anti-NOS3 / eNOS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $425.00
NOS3 / eNOS Antibody - Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Select
Rabbit Polyclonal to Human NOS3 / eNOS
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µg   1mg/ml   $495.00
NOS3 / eNOS Antibody - Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Mouse Monoclonal (IgG1) to Human NOS3 / eNOS
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
NOS3 / eNOS Antibody - Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
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Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
NOS3 / eNOS Antibody - IHC using eNOS Antibody
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Guinea pig, Sheep, Rat, Horse, Bat, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   $425.00
NOS3 / eNOS Antibody
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
NOS3 / eNOS Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
Rabbit Polyclonal to Human NOS3 / eNOS
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl   $325.00
NOS3 / eNOS Antibody - Immunohistochemical analysis of eNOS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human NOS3 / eNOS
Rabbit, Mouse, Dog, Sheep, Bovine, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $275.00
NOS3 / eNOS Antibody - Western blot of eNOS antibody
Select
Mouse Monoclonal [clone Mix] to Human NOS3 / eNOS
Mouse, Rat, Human
WB
Unconjugated
50µl   $235.00; 100µl   $265.00; 200µl   $335.00
Select
Rabbit Polyclonal (IgG) to Human NOS3 / eNOS
Mouse, Dog, Bovine, Rat, Human
WB
Unconjugated
100µl   $710.00

Viewing 1-25 of 207 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).