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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C661983-100 100 µg $325 
Leptin Antibody - Western blot analysis of Leptin using anti-Leptin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat Leptin protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD.

Leptin Antibody LS‑C661983

Leptin Antibody LS‑C661983

Antibody:
Rabbit Polyclonal to Rat Leptin
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C661983-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rat Leptin
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Leptin antibody LS-C661983 is an unconjugated rabbit polyclonal antibody to rat Leptin. Validated for ELISA and WB.
Target
Rat Leptin
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively.
Presentation
5mg BSA 0.9mg NaCl 0.2mg Na2HPO4 0.05mg NaN3 per 100ug antibody
Reconstitution
Reconstitute with 0.2ml distilled H2O.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Leptin
P41159 NM_000230 NP_000221.1

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Requested From: United States
Date Requested: 1/27/2020