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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B6182-50 50 µl (1 mg/ml) $485 
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHCPlus™ Polyclonal HIF1A / HIF1 Alpha Antibody (IHC, WB) LS‑B6182

IHCPlus™ Polyclonal HIF1A / HIF1 Alpha Antibody (IHC, WB) LS‑B6182

Note: This antibody replaces LS-C7466
Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B6182-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HIF1 Alpha antibody LS-B6182 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) from human and rat. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein of human HIF-1 alpha.
Specificity
Specific for HIF-1 alpha. Species cross-reactivity: Human and rat.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot:1:500-1:1000 (ECL). ChIP: 25 ul antibody for 15 million cells. Positive control: PC12 nuclear extracts.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-HIF1A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-HIF1A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ HIF1A / HIF1 Alpha Antibody for IHC, WB/Western, IP LS-B6182 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/10/2020