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IHCPlus™ HIF1A / HIF1 Alpha Antibody aa530‑825 Polyclonal IHC LS‑B495

Catalog Number Size Price
LS-B495-100 100 µl (1 mg/ml) $425 
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ HIF1A / HIF1 Alpha Antibody aa530‑825 Polyclonal IHC LS‑B495

Note: This antibody replaces LS-C2558
Antibody:
Rabbit Polyclonal to Mouse HIF1A / HIF1 Alpha
Application:
IHC, IHC-P
Reactivity:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B495-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse HIF1A / HIF1 Alpha
Application:
IHC, IHC-P
Reactivity:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HIF1 Alpha antibody LS-B495 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A ) from human, mouse, rat and other species. Validated for IHC. Cited in 5 publications.
Target
Mouse HIF1A / HIF1 Alpha
Host
Rabbit
Reactivity
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
Epitope
aa530-825
Specificity
HIF-1 alpha.
Usage
Immunohistochemistry: LS-B495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B495 was determined to be 5-10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (5)

Carbon ion radiotherapy of human lung cancer attenuates HIF-1 signaling and acts with considerably enhanced therapeutic efficiency. Subtil FS, Wilhelm J, Bill V, Westholt N, Rudolph S, Fischer J, Scheel S, Seay U, Fournier C, Taucher-Scholz G, Scholz M, Seeger W, Engenhart-Cabillic R, Rose F, Dahm-Daphi J, H[Character e4]nze J. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Mar;28:1412-21.

HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Malec V, Gottschald OR, Li S, Rose F, Seeger W, H[Character e4]nze J. Free radical biology & medicine. 2010 Jun;48:1626-35.

Loss of HIF-1 alpha in the notochord results in cell death and complete disappearance of the nucleus pulposus. Merceron C, Mangiavini L, Robling A, Wilson TL, Giaccia AJ, Shapiro IM, Schipani E, Risbud MV. PloS one. 9:e110768.

ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment. Zhu K, Jiao H, Li S, Cao H, Galson DL, Zhao Z, Zhao X, Lai Y, Fan J, Im HJ, Chen D, Xiao G. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013 28:1870-1884. (IHC; Mouse)

Hypoxia-driven Hif2a coordinates mouse liver regeneration by coupling parenchymal growth to vascular expansion. Kron P, Linecker M, Limani P, Schlegel A, Kambakamba P, Lehn JM, Nicolau C, Graf R, Humar B, Clavien PA. Hepatology (Baltimore, Md.). 2016 December;64:2198-2209. (Mouse)

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Requested From: United States
Date Requested: 9/16/2019