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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B495-100 100 µl (1 mg/ml) $425 
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (aa530‑825, IHC) LS‑B495

IHCPlus™ Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (aa530‑825, IHC) LS‑B495

Note: This antibody replaces LS-C2558
Antibody:
Rabbit Polyclonal to Mouse HIF1A / HIF1 Alpha
Application:
IHC, IHC-P
Reactivity:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B495-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse HIF1A / HIF1 Alpha
Application:
IHC, IHC-P
Reactivity:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HIF1 Alpha antibody LS-B495 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC. Cited in 5 publications.
Target
Mouse HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
Epitope
aa530-825
Specificity
HIF-1 alpha.
Usage
Immunohistochemistry: LS-B495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B495 was determined to be 5-10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa530-825) for IHC LS-B495 has an LSBio Rating of
Publications (4.4)
Laboratory Validation Score (4)

Publications (5)

Carbon ion radiotherapy of human lung cancer attenuates HIF-1 signaling and acts with considerably enhanced therapeutic efficiency. Subtil FS, Wilhelm J, Bill V, Westholt N, Rudolph S, Fischer J, Scheel S, Seay U, Fournier C, Taucher-Scholz G, Scholz M, Seeger W, Engenhart-Cabillic R, Rose F, Dahm-Daphi J, H[Character e4]nze J. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Mar;28:1412-21.

HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Malec V, Gottschald OR, Li S, Rose F, Seeger W, H[Character e4]nze J. Free radical biology & medicine. 2010 Jun;48:1626-35.

Loss of HIF-1 alpha in the notochord results in cell death and complete disappearance of the nucleus pulposus. Merceron C, Mangiavini L, Robling A, Wilson TL, Giaccia AJ, Shapiro IM, Schipani E, Risbud MV. PloS one. 9:e110768.

ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment. Zhu K, Jiao H, Li S, Cao H, Galson DL, Zhao Z, Zhao X, Lai Y, Fan J, Im HJ, Chen D, Xiao G. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013 28:1870-1884. (IHC; Mouse)

Hypoxia-driven Hif2a coordinates mouse liver regeneration by coupling parenchymal growth to vascular expansion. Kron P, Linecker M, Limani P, Schlegel A, Kambakamba P, Lehn JM, Nicolau C, Graf R, Humar B, Clavien PA. Hepatology (Baltimore, Md.). 2016 December;64:2198-2209. (Mouse)

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Requested From: United States
Date Requested: 7/10/2020