Products
Services
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B1503-1 1 ea $425 
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1A / HIF1 Alpha antibody.
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1A / HIF1 Alpha antibody.
1 of 2
2 of 2

IHCPlus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa833‑850, IHC, WB) LS‑B1503

IHCPlus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa833‑850, IHC, WB) LS‑B1503

Note: This antibody replaces LS-C11695
Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1503-1
1 ea
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
HIF1 Alpha antibody LS-B1503 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa833-850) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HIF-1 amino acids 809-826. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%); Turkey, Chicken, Platypus (94%); Opossum, Lizard, Xenopus (89%); Pike, Zebrafish (83%).
Blocking Peptide
LS-E47595 - Lyophilized - 1 ea - $145.00
Immunizing peptide used to generate LS-B1503. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa833-850
Specificity
HIF-1 alpha amino acids (SRNLLQGEELLRALDQVN)
Usage
Immunohistochemistry: LS-B1503 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1503 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.2, 0.02% Sodium Azide, 50% Glycerol
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa833-850) for IHC, WB/Western LS-B1503 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Loss of PINK1 attenuates HIF-1 alpha induction by preventing 4E-BP1-dependent switch in protein translation under hypoxia. Lin W, Wadlington NL, Chen L, Zhuang X, Brorson JR, Kang UJ. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2014 Feb;34:3079-89.

Customer Reviews (0)


Requested From: United States
Date Requested: 7/10/2020