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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B110-100 100 µl (1 mg/ml) $425 
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone H1alpha67, aa432‑528, IHC, WB) LS‑B110

IHCPlus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone H1alpha67, aa432‑528, IHC, WB) LS‑B110

Note: This antibody replaces LS-C2556, LS-C7462
Antibody:
Mouse Monoclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, ELISA, ChrIP
Reactivity:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B110-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, ELISA, ChrIP
Reactivity:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HIF1 Alpha antibody LS-B110 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa432-528) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, ELISA, Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
H1alpha67
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:20 - 1:50)
  • Western blot (1:500)
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA
  • Chromatin Immunoprecipitation (1:10 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1 alpha. [UniProt# Q16665]
Epitope
aa432-528
Specificity
HIF-1 alpha.
Usage
Immunohistochemistry: LS-B110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B110 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 1% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528, clone H1alpha67) for IHC, WB/Western, IP, Flow, ELISA LS-B110 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/3/2020