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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C166867-400 400 µl $325 
HIF1A / HIF1 Alpha Antibody - HIF1Alpha Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody for immunohistochemistry.
HIF1A / HIF1 Alpha Antibody - Confocal immunofluorescence of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1Alpha Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified antibody.
HIF1A / HIF1 Alpha Antibody - HIF1Alpha Antibody flow cytometry of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HIF1A / HIF1 Alpha Antibody - HIF1Alpha Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody for immunohistochemistry.
HIF1A / HIF1 Alpha Antibody - Confocal immunofluorescence of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1Alpha Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified antibody.
HIF1A / HIF1 Alpha Antibody - HIF1Alpha Antibody flow cytometry of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa519‑547, IHC, IF, WB) LS‑C166867

Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa519‑547, IHC, IF, WB) LS‑C166867

Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C166867-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HIF1A / HIF1 Alpha
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HIF1 Alpha antibody LS-C166867 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa519-547) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9242 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C166867. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa519-547
Specificity
This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

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Requested From: United States
Date Requested: 7/4/2020