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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIF1A / HIF1 Alpha Antibody (aa432‑528, clone H1alpha67) LS‑C2557
HIF1 Alpha antibody LS-C2557 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) from human, mouse, rat and other species. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C2557-100
100 µl (2 mg/ml)
$335
Description
HIF1 Alpha antibody LS-C2557 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) from human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human HIF1A / HIF1 Alpha
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog, Ferret, Pig, Sheep, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [H1alpha67]
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, HiLyte Fluor 488, HiLyte Fluor, DY550.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:1000)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1 alpha
Epitope
aa432-528
Specificity
HIF-1 alpha.
Presentation
Tris-Glycine, pH 7.5, 150 mM NaCl, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

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Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation

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Images

Immunohistochemistry

Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunoprecipitation of HIF-1alpha. Heavy and light chains are also detected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot analysis, anti-HIF-1 alpha, on cobalt chloride untreated/treated Cos 7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/17/2018
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