Human GRN / Granulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhGRN.

Recognizes human GRN. In direct ELISAs and Western Blots the antibody showed no cross reactivity with recombinant mouse progranulin.

• Western blot (1 - 2 µg/ml)
• Immunoprecipitation (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 ug/ml; The detection limit for rhGRN is approximately 2 ng/well. Western Blot: 1-2 ug/ml; Using a colorimetric detection system, the detection limit for rhGRN is ~5 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 ug/ml to immunoprecipitate rhGRN from conditioned cell culture medium. The recovered rhGRN can be detected in Western Blots using an affinity-purified polyclonal antibody.

Lyophilized from PBS, pH 8.0, 20mM Tris, 300mM sodium chloride, 5% trehalose

Reconstitute in 200 ul sterile PBS

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P28799 NCBI: NM_002087 NP_002078.1