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IHCPlus™ GRN / Granulin Antibody C‑Terminus Polyclonal IHC,IP,WB LS‑B3158

Catalog Number Size Price
LS-B3158-50 50 µg (0.25 mg/ml) $485 
Anti-GRN / Granulin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ GRN / Granulin Antibody C‑Terminus Polyclonal IHC,IP,WB LS‑B3158

Note: This antibody replaces LS-C17498
Antibody:
Rabbit Polyclonal to Human GRN / Granulin
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B3158-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GRN / Granulin
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Granulin antibody LS-B3158 is an unconjugated rabbit polyclonal antibody to human Granulin (GRN ). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GRN / Granulin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the C-terminal region of human PCDGF (PC cell-derived growth factor, GP88, acrogranin, granulin precursor, epithelin precursor, or progranulin)
Epitope
C-Terminus
Specificity
Recognizes human PCDGF protein at ~64-88kD.
Usage
Immunohistochemistry: LS-B3158 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3158 was determined to be 5 ug/ml. Positive control: Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GRN / Granulin
P28799 NM_002087 NP_002078.1

Validation

Anti-GRN / Granulin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-GRN / Granulin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GRN / Granulin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Species: Human
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Species: Mouse
Applications: ELISA
GRN antibody (1 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western Blot; Sample: Mouse Kidney lysate; Primary Ab: 2µg/ml Rabbit Anti-Mouse GRN Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Species: Mouse
Applications: Western blot
GRN antibody (1 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Range: 50-1000 pg/ml

Requested From: United States
Date Requested: 9/16/2019