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GRN / Granulin Antibody (aa248‑259) LS‑C154960

Catalog Number Size Price
LS-C154960-100 100 µg (0.5 mg/ml) $405 
GRN antibody (1 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GRN / Granulin Antibody (aa248‑259) LS‑C154960

Antibody:
Goat Polyclonal to Human GRN / Granulin
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C154960-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human GRN / Granulin
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
Granulin antibody LS-C154960 is an unconjugated goat polyclonal antibody to Granulin (GRN ) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Target
Human GRN / Granulin
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Immunogen
Peptide with sequence PQDTVCDLIQSK, from the internal of the protein sequence according to NP_002078.1.
Blocking Peptide
LS-E27830 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154960. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa248-259
Specificity
Human GRN / Granulin. This antibody is expected to recognize the C-Terminal part of Granulin-3.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 75kD band observed in lysates of cell lines A431, A549 and HeLa (calculated MW of 63.5kD according to NP_002078.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (He and Bateman, Cancer Res. 1999 Jul 1;59(13):3222-9. PMID: 10397269). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GRN / Granulin
P28799 NM_002087 NP_002078.1

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Requested From: United States
Date Requested: 9/21/2019