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Catalog Number Size Price
LS-C50830-250 250 µg $702 
Antibody

Polyclonal Chicken anti‑Aequorea victoria GFP Antibody (WB) LS‑C50830

Polyclonal Chicken anti‑Aequorea victoria GFP Antibody (WB) LS‑C50830

Antibody:
GFP Chicken anti-Aequorea victoria Polyclonal Antibody
Application:
ICC, WB, IP
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$702
LS-C50830-250
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GFP Chicken anti-Aequorea victoria Polyclonal Antibody
Application:
ICC, WB, IP
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GFP antibody LS-C50830 is an unconjugated chicken polyclonal antibody to aequorea victoria GFP. Validated for ICC, IP and WB.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Chicken
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Immunogen
Affinity purified, recombinant green fluorescent protein (GFP) containing a 6-His tag (single band, SDS-PAGE).
Specificity
Recognizes green fluorescent protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts.
Applications
  • ICC
  • Western blot
  • Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Western Blot: Detects a band of the proper molecular weight, 30kD, in lysates from E. coli expressing GFP, but not in E. coli that do not express or in lysates prepared from E. coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50 ug of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 1:5000-1:10000 when detected with an anti-chicken HRP conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. Binding was determined using HRP conjugated, anti-chicken secondary antibodies and visualized using DAB. The anti-GFP antibodies were used at dilutions of 1:1000-1:2500. Cells were fixed using 4% formaldehyde in PBS, pH 7.4. For immunocytochemistry, the cultures were fixed in 4% formaldehyde in PBS, pH 7.4, for 20 minutes at RT. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4?C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes. Has been used to detect GFP in primary neurons and zebrafish fibroblasts.
Presentation
10 mM sodium chloride, 0.02% sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022