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GFP Antibody (clone N/K) LS‑C58894

Catalog Number Size Price
LS-C58894-0.1 0.1 ml $575 
Antibody

GFP Antibody (clone N/K) LS‑C58894

Antibody:
Mouse Monoclonal to GFP
Application:
IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Price
Catalog Number
$575 
LS-C58894-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to GFP
Application:
IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified

Specifications

Description
GFP antibody LS-C58894 is an unconjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
N/K
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (1:500 - 1:1000)
  • IHC - Frozen (1:500 - 1:1000)
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GFP-BSA conjugate.
Specificity
Recognizes various forms of green fluorescent protein including eGFP and those forms found in vectors supplied by Clontech and Invitrogen. The antibody does not cross react with RFP. Cross reactivity with YFP has not been tested.
Presentation
PBS, BSA
Storage
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
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Product
Species: Aequorea victoria
Applications: IHC, Western blot, ELISA
Reactivity: All species
Range: 15.6-1000 pg/ml

Requested From: United States
Date Requested: 9/15/2019